Roles for C-X-C chemokines and C5a in lung injury after hindlimb ischemia-reperfusion.
Am J Physiol
; 276(1): L57-63, 1999 01.
Article
em En
| MEDLINE
| ID: mdl-9887056
ABSTRACT
We evaluated the roles of the C-X-C chemokines cytokine-induced neutrophil chemoattractant (CINC) and macrophage inflammatory protein-2 (MIP-2) as well as the complement activation product C5a in development of lung injury after hindlimb ischemia-reperfusion in rats. During reperfusion, CD11b and CD18, but not CD11a, were upregulated on neutrophils [bronchoalveolar lavage (BAL) and blood] and lung macrophages. BAL levels of CINC and MIP-2 were increased during the ischemic and reperfusion periods. Treatment with either anti-CINC or anti-MIP-2 IgG significantly reduced lung vascular permeability and decreased lung myeloperoxidase content by 93 and 68%, respectively (P < 0.05). During the same period, there were significant increases in serum C5a-related neutrophil chemotactic activity. Treatment with anti-C5a decreased lung vascular permeability, lung myeloperoxidase, and BAL CINC by 51, 58, and 23%, respectively (P < 0.05). The data suggest that the C-X-C chemokines CINC and MIP-2 as well as the complement activation product C5a are required for lung neutrophil recruitment and full induction of lung injury after hindlimb ischemia-reperfusion in rats.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Traumatismo por Reperfusão
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Complemento C5a
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Quimiocinas
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Quimiocinas CXC
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Peptídeos e Proteínas de Sinalização Intercelular
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Membro Posterior
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Isquemia
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Pulmão
Limite:
Animals
Idioma:
En
Revista:
Am J Physiol
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Alemanha