Cell ashing for trace element analysis: A new approach based on ultraviolet/ozone.
Anal Biochem
; 266(2): 174-80, 1999 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-9888973
ABSTRACT
We studied a new approach to cell ashing based on illuminating the specimens with a low-pressure mercury discharge lamp. We analyzed with synchrotron spectromicroscopy its effects on different physiological elements in neurobiological specimens. Our results demonstrate that carbon is removed, whereas phosphorus, calcium, potassium, and sulfur are retained and their relative concentrations are enhanced. Applied to trace elements, this technique will enhance their practical detectability.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligoelementos
/
Técnicas de Química Analítica
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
1999
Tipo de documento:
Article