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Antigen-dependent and -independent IFN-gamma modulation by penicillins.
Padovan, E; von Greyerz, S; Pichler, W J; Weltzien, H U.
Afiliação
  • Padovan E; Max Planck Institute for Immunobiology, Freiburg, Germany.
J Immunol ; 162(2): 1171-7, 1999 Jan 15.
Article em En | MEDLINE | ID: mdl-9916749
The activation of CD4+ T lymphocytes upon Ag stimulation plays a critical role in adverse immune responses including drug-specific hypersensitivity reactions. We examined the modulation of T cell phenotype induced by hapten-specific stimulation using the model of beta-lactam antibiotics such as penicillin G (Pen G), Pen V, and ampicillin (Amp). When PBMC of donors suffering from hypersensitivity reactions against beta-lactams were stimulated in vitro with different doses of Pen G, a preferential expansion of IL-4-producing TCR alphabeta+ cells was detected. A panel of T cell clones was then prepared from Pen G-specific lines after two cycles of restimulation with the hapten. For the majority of these clones, we found that high doses of Pen G induced optimal IL-4 secretion, whereas the amount of IFN-gamma secreted was inversely correlated with the dose of Pen G, thus leading to a hapten-inducible shift of the functional phenotypes for some of the clones. Finally, Pen V and Amp were used to modulate different Ag-induced immune responses. We found that Amp had no influence on the cytokine pattern induced by specific Ag or mitogens. In contrast, Pen V inhibited the secretion of IFN-gamma, but not IL-4, most likely by Ag-independent mechanisms. This last finding may open new applications for immune intervention in those diseases in which polarized Th1 responses are involved in the development of the pathology.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Penicilinas / Interferon gama / Antígenos Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Immunol Ano de publicação: 1999 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Estados Unidos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Penicilinas / Interferon gama / Antígenos Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Immunol Ano de publicação: 1999 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Estados Unidos