Why superoxide imposes an aromatic amino acid auxotrophy on Escherichia coli. The transketolase connection.
J Biol Chem
; 274(7): 4202-6, 1999 Feb 12.
Article
em En
| MEDLINE
| ID: mdl-9933617
ABSTRACT
The lack of superoxide dismutase and the consequent elevation of [O2-] imposes, on Escherichia coli, auxotrophies for branched chain, sulfur-containing, and aromatic amino acids. The former two classes of auxotrophies have already been explained, whereas the third is explained herein. Thus O2- is shown to interfere with the production of erythrose-4-phosphate, which is essential for the first step of the aromatic biosynthetic pathway. It does so by oxidizing the 1, 2-dihydroxyethyl thiamine pyrophosphate intermediate of transketolase and inactivating this enzyme.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Superóxido Dismutase
/
Proteínas de Bactérias
/
Transcetolase
/
Superóxidos
/
Escherichia coli
Idioma:
En
Revista:
J Biol Chem
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Estados Unidos