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Comparison of two human pathogenic strains with a commensal strain of Herpes Simplex virus isolated at the University Hospital of the West Indies
Kingston; s.n; 1972. ix,185 p. ills, tab.
Tese em Inglês | MedCarib | ID: med-13716
Biblioteca responsável: JM23.1
Localização: JM23.1; U Thesis
ABSTRACT
Comparision has been made between two human "neuropathogenic" and one "commensal" strains of herpes simplex virus. These terms are assigned to these viruses on the basis of their origin. The first two were related from patients with encephalitis and the third from a benign recurrent lesion. A number of physiochemical characteristics of these three strains were studied. The major differences observed between the "pathogens" and the "commensal" virus are 1. The ability of the commensal herpes virus to infect adult mice while the pathogenic herpes could not. 2. The commensal herpes virus could not be neutralized by the hyperimmune sera of the pathogenic herpes and vice versa, although all crossreacted in complement fixation tests. 3. The commensal virus was significantly inactivated by chloroform, while the pathogens were not significantly affected under the same conditions. 4. Freezing and thawing followed by differential centrifugation rendered the commensal herpes vulnerable to inactivation of DNAse. Inactivation of the pathogens by this enzyme could only be acheived after chromatography on the anion exchanger, Diethyl aminoethyl. 5. The absorbtion spectrum gave a maximum peak at wave lenghts between 260-270 mu for the pathogenic virus. The peak absorption of the commensal herpes was at 280 mu. The peak at 260-270 mu by the pathogens is most likely due to the presence of nucleic acid. The peak absorption of the commensal virus is similar to that given by a typical protein. The relatively high concentration of protein which accompanies the commensal virus might account for the behaviour of this virus. 6. The "pathogenic" and "commensal" strains could be distinguished with respect to susceptibility to interferon. The commensal strain being more susceptible and also more effective in inducing interferon synthesis. 7. The method of comparision used in this investigation, and the results which reflect the marked difference between the strains of herpes simplex studied here produce some methods which may be used for differentiating between pathogenic and commensal strains of herpes simplex virus. It would appear that the methods used here are valuable to the study of viral genetics and the physiochemical study of herpes viruses. Evidence has been presented to show that there is molecular structural difference between the pathogenic and the commensal strains of herpes simplex virus. Such a difference or such differences may provide tools to probe into the pathogenic physiology of infected cells, or help to elucidate the factors responsible for neurotropicity and commensalism in herpes virus. The author is convinced that the differences cited above are genetically based. However, until further investigations are carried out, it is a subjective choice whether to consider the differences as sufficient basis for the subgrouping of herpes simplex virus. No claim is made that the distinction cited are attributes of other strains associated with encephalitis or limited lesions of recurrent herpes (AU)
Assuntos
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Coleções: Bases de dados internacionais Base de dados: MedCarib Assunto principal: Simplexvirus Limite: Adulto / Animais / Criança / Criança, pré-escolar / Feminino / Humanos / Masculino País/Região como assunto: Caribe / Caribe Inglês / Jamaica Idioma: Inglês Ano de publicação: 1972 Tipo de documento: Tese
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Coleções: Bases de dados internacionais Base de dados: MedCarib Assunto principal: Simplexvirus Limite: Adulto / Animais / Criança / Criança, pré-escolar / Feminino / Humanos / Masculino País/Região como assunto: Caribe / Caribe Inglês / Jamaica Idioma: Inglês Ano de publicação: 1972 Tipo de documento: Tese
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