Control externo de la calidad del diagnostico serologico del dengue en laboratorios de paises de las Americas, 1996-2001 / Quality control of the serological diagnosis of dengue in laboratories throughout the Americas, 1996-2001

ABSTRACT

Objective. To report the results from participating laboratories for four external quality control proficiency tests of dengue serological diagnosis that were carried out in the Region of the Americas in the period of 1996-2001. Methods. External quality control proficiency tests of dengue serological diagnosis were carried out in 1996-1997, 1998-1999, 2000-2001. Panels made up of 20 serum samples (12 of them positive for dengue IgM antibodies) were sent to participating laboratories in the Region. The sera were negative for HIV antibodies, hepatitis C virus antibodies, and hepatitis B surface antigen. The sera were stored at -20 degrees C until they were sent in refrigerated shipments to the participating laboratories. THe presence of IgM antibodies was determined through IgM-capture enzyme-linked immunosorbent assay (ELISA), while the IgG antibody titer was determined by hemagglutination inhibition or by IgG ELISA. The results of the IgM antibody testing that differed from those of the reference center were considered discordant. The IgG antibody titer was considered discordant when the results differed by two dilutions or more with respect to the reference center's results. Results. A total of 27 laboratories received a total of 59 serum panels over the 1996-2001 period, and the results from testing 54 of those panels (91.5 percent) were sent back in. Of the total of 1, 080 sera samples from those 54 panels, the results from 95.6 percent of the IgM antibody tests were concordant with the results from the reference center. With 47 of the 54 panels (87.0 percent) the participating laboratories' agreement with the reference center's results for the IgM antibody testing was 90.0 percent or higher. The laboratories sent back results from a total of 27 IgG antibody titer tests, and 22 of them (81.5 percent) coincided with those from the reference center. Considering the IgM antibody testing results from the four periods, the findings from 22 of the participating laboratories coincided with those from the reference center for at least 90 percent of the samples, and 13 of the laboratories were in complete concordance with the reference center. Conclusions. The majority of the participating laboratories showed an excellent level of performance in detecting dengue IgG and IgM antibodies. However, the deficiencies found in some instances confirm the need for continuing to improve laboratory diagnosis of dengue in the Region of the Americas (AU)