In-house polymerase chain reaction for affordable and sustainable Chlamydia trachomatis detection in Trinidad and Tobago.
Rev. panam. salud pública
; 22(5): 317-322, Nov 2007. tabilus
Artigo
em Inglês
| MedCarib
| ID: med-17742
Biblioteca responsável:
TT5
Localização: TT5; W1, RE712AW
ABSTRACT
OBJECTIVES:
To provide a preliminary assessment of in-house polymerase chain reaction (PCR) as an alternative to the more costly commercial test for detection of asymptomatic infection by Chlamydia trachomatis and to provide much needed demographic data on infection indicators within the Trinidad and Tobago public health care system.METHODS:
An inexpensive in-house nested-PCR with an Internal Amplification Control was used to detect C. trachomatis and Neisseria gonorrhoeae in urine samples collected from 273 apparently healthy, pregnant women from March-September 2004 in Trinidad, West Indies. Demographic information on participants was collected and subjected to statistical analyses.RESULTS:
C. trachomatis was detected in 57/273 (21 per cent) samples, of which 5 (2 per cent) were also positive for N. gonorrhoeae. Infection correlated well with certain demographic parameters, with the highest incidence of C. trachomatis infection found among pregnant women that were single or of African descent.CONCLUSIONS:
Given the lack of commercial tests in Trinidad, in-house PCR is an inexpensive alternative that can be used to detect asymptomatic infections of C. trachomatis and to provide demographic information needed for interventions by the public health care system.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
MedCarib
Assunto principal:
Trinidad e Tobago
/
Chlamydia trachomatis
/
Reação em Cadeia da Polimerase
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
País/Região como assunto:
Caribe Inglês
/
Trinidad e Tobago
Idioma:
Inglês
Revista:
Rev. panam. salud pública
Ano de publicação:
2007
Tipo de documento:
Artigo
Instituição/País de afiliação:
The University of the West Indies/Trinidad and Tobago
/
University of Texas/United States of America