The effect of S-nitroso-glutathione on insulin receptors on erythrocytes abd mononuclear leucocytes in dogs

ABSTRACT

This study was designed to investigate differences in cellular binding of insulin in dogs administered with the nitric oxide donor, S-nitroso-glutathione (GSNO). A time course assay of insulin binding to isolated erythrocytes and mononuclear leucocytes from dogs administered with GSNO was done during the oral glucose tolerance test (OGTT). The erythrocyte receptor assay performed was the methodology used by Ghambir et al (1977). A modification was also done for mononuclear leucocyte insulin binding assay. The plasma glucose levels were measured by the glucose oxidase method, while the insulin levels were determined by radioimmunoassay. The results of these studies show the erythrocytes and mononuclear leucocytes from dogs administered with GSNO have lesser ability to bind insulin when compared to erythrocytes and mononuclear leucocytes from the controls. In dogs administered with GSNO, there was less binding of erythrocytes and mononuclear leucocytes, 44 percent and 29 percent, respectively, when compared to the bound free ratio value of the controls.(AU)