Restriction site polymorphisms at the human HepG2 glucose transporter gene locu in Caucasian and West Indian subjects with non-insulin-dependent diabetes mellitus

ABSTRACT

Digestion of human genomic DNA with the restriction enzyme Stul revealed a 2-allele polymorphism with a human HepG2 glucose transporter probe. Bands of 3.2 kilobases (kb; SI allele) and 2.6 kb (S2 allele) were observed. The genotype frequencies were investigated in 2 non-insulin-dependent diabetic populations. The gene type frequencies of S1S1, S1S2 and S2S2 were 6, 42 and 52 percent among Caucasian diabetic subjects (n=48), and 11, 38 and 51 percent in 47 controls, respectively. In West Indian diabetic patients (n=48), the genotype frequencies were 17, 54 and 29 percent, and for 36 controls they were 25, 33 and 42 percent, respectively. The polymorphism information content of this restriction fragment length polymorphism (RFLP) is 0.32 in Caucasians and 0.37 in West Indians, respectively. There was no significant difference of allele or genotype frequencies between the diabetic patients and non-diabetic controls in either group. Haple type analysis of Stul and Xbal RFPLs showed that there was also no significant difference in the frequencies of the four different haplotypes S1X1, S1X2, S2X1 and S2X2 between the patients and controls. However there was a difference for the frequency of the S1 allele between Caucasians (control 30 percent, patients 27 percent) and West Indians (controls 42 percent, patients 44 percent). There was also a significant difference in the frequency of haplotype S2X2 between these two racial groups (controls 48 percent, cases 51 percent for Caucasians, and controls 33 percent, cases 22 percent for West Indians). (AU)