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Distribution of ACE2, CD147, cyclophilins, CD26 and other SARS-CoV-2 associated molecules in human tissues and immune cells in health and disease
Urszula Radzikowska; Mei Ding; Ge Tan; Damir Zhakparov; Yaqi Peng; Paulina Wawrzyniak; Ming Wang; Shuo Li; Hideaki Morita; Can Altunbulakli; Matthias Reiger; Avidan U Neumann; Nonhlanhla Lunjani; Claudia Traidl-Hoffmann; Kari Nadeau; Cezmi A Akdis; Milena Sokolowska.
Afiliação
  • Urszula Radzikowska; Swiss Institute of Allergy and Asthma Research, University of Zurich and Christine-Kuhne - Center for Research and Education, Davos, Switzerland; Department of
  • Mei Ding; Swiss Institute of Allergy and Asthma Research, University of Zurich and Christine-Kuhne - Center for Research and Education, Davos, Switzerland; Department of
  • Ge Tan; Swiss Institute of Allergy and Asthma Research, University of Zurich, Davos, Switzerland; Functional Genomic Centre Zurich, ETH Zurich/University of Zurich, Zur
  • Damir Zhakparov; Swiss Institute of Allergy and Asthma Research, University of Zurich, Davos, Switzerland
  • Yaqi Peng; Swiss Institute of Allergy and Asthma Research, University of Zurich and Christine-Kuhne - Center for Research and Education, Davos, Switzerland; Otorhinolaryng
  • Paulina Wawrzyniak; Swiss Institute of Allergy and Asthma Research, University of Zurich and Christine-Kuhne - Center for Research and Education, Davos, Switzerland; Div. od Clinic
  • Ming Wang; Swiss Institute of Allergy and Asthma Research, Univ. of Zurich and Christine-Kuhne - Center for Research and Education, Davos, Switzerland; Department of Otola
  • Shuo Li; Swiss Institute of Allergy and Asthma Research, University of Zurich, Davos, Switzerland; Department of Cancer Immunology, Institute for Cancer Research, Oslo U
  • Hideaki Morita; Swiss Institute of Allergy and Asthma Research, University of Zurich, Davos, Switzerland; Department of Allergy and Clinical Immunology, National Research Insti
  • Can Altunbulakli; Swiss Institute of Allergy and Asthma Research, University of Zurich and Christine-Kuhne - Center for Research and Education, Davos, Switzerland
  • Matthias Reiger; Chair and Institute of Environmental Medicine, UNIKA-T, Technical University of Munich and Helmholtz Zentrum Munchen, Augsburg, Germany
  • Avidan U Neumann; Chair and Institute of Environmental Medicine, UNIKA-T, Technical University of Munich and Helmholtz Zentrum Munchen, Augsburg, Germany; Institute of Computiona
  • Nonhlanhla Lunjani; Swiss Institute of Allergy and Asthma Research, University of Zurich and Christine-Kuhne - Center for Research and Education, Davos, Switzerland
  • Claudia Traidl-Hoffmann; Christine-Kuhne - Center for Research and Education, Davos, Switzerland; Chair and Institute of Environmental Medicine, UNIKA-T, Technical University of Munich
  • Kari Nadeau; Sean N Parker Centre for Allergy and Asthma Research at Stanford University, Department of Medicine, Stanford University School of Medicine, Stanford, USA
  • Cezmi A Akdis; Swiss Institute of Allergy and Asthma Research, University of Zurich and Christine-Kuhne - Center for Research and Education, Davos, Switzerland
  • Milena Sokolowska; Swiss Institute of Allergy and Asthma Research, University of Zurich and Christine-Kuhne - Center for Research and Education, Davos, Switzerland
Preprint em En | PREPRINT-BIORXIV | ID: ppbiorxiv-090332
ABSTRACT
BackgroundMorbidity and mortality from COVID-19 caused by novel coronavirus SARS-CoV-2 is accelerating worldwide and novel clinical presentations of COVID-19 are often reported. The range of human cells and tissues targeted by SARS-CoV-2, its potential receptors and associated regulating factors are still largely unknown. The aim of our study was to analyze the expression of known and potential SARS-CoV-2 receptors and related molecules in the extensive collection of primary human cells and tissues from healthy subjects of different age and from patients with risk factors and known comorbidities of COVID-19. MethodsWe performed RNA sequencing and explored available RNA-Seq databases to study gene expression and co-expression of ACE2, CD147 (BSG), CD26 (DPP4) and their direct and indirect molecular partners in primary human bronchial epithelial cells, bronchial and skin biopsies, bronchoalveolar lavage fluid, whole blood, peripheral blood mononuclear cells (PBMCs), monocytes, neutrophils, DCs, NK cells, ILC1, ILC2, ILC3, CD4+ and CD8+ T cells, B cells and plasmablasts. We analyzed the material from healthy children and adults, and from adults in relation to their disease or COVID-19 risk factor status. ResultsACE2 and TMPRSS2 were coexpressed at the epithelial sites of the lung and skin, whereas CD147 (BSG), cyclophilins (PPIA and PPIB), CD26 (DPP4) and related molecules were expressed in both, epithelium and in immune cells. We also observed a distinct age-related expression profile of these genes in the PBMCs and T cells from healthy children and adults. Asthma, COPD, hypertension, smoking, obesity, and male gender status generally led to the higher expression of ACE2- and CD147-related genes in the bronchial biopsy, BAL or blood. Additionally, CD147-related genes correlated positively with age and BMI. Interestingly, we also observed higher expression of ACE2- and CD147-related genes in the lesional skin of patients with atopic dermatitis. ConclusionsOur data suggest different receptor repertoire potentially involved in the SARS-CoV-2 infection at the epithelial barriers and in the immune cells. Altered expression of these receptors related with age, gender, obesity and smoking, as well as with the disease status might contribute to COVID-19 morbidity and severity patterns.
Licença
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Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-BIORXIV Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Preprint
Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-BIORXIV Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Preprint