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Previously unrecognized non-reproducible antibody-antigen interactions and their implications for diagnosis of viral infections including COVID-19
Jiaojiao Pan; Lan Yang; Yi Deng; Baoqing Sun; Li Zhang; Wenya Wu; Jingzhi Li; Hu Cheng; Yiting Li; Wenwen Xu; Jiao Yang; Yiyue Sun; Hao Fei; Qinghong Xue; Youxin Zhou; Hui Wang; Peiyan Zheng; Hao Chen; Feng Cai Zhu; Daxin Peng; Jun Han; Jiwan Qiu; Hongwei Ma.
Afiliação
  • Jiaojiao Pan; Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
  • Lan Yang; Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
  • Yi Deng; Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
  • Baoqing Sun; Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, The First Affiliated Hosp
  • Li Zhang; Department of Vaccine Clinical Evaluation, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing, 210009, China.
  • Wenya Wu; Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
  • Jingzhi Li; Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
  • Hu Cheng; Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
  • Yiting Li; Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
  • Wenwen Xu; Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
  • Jiao Yang; Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
  • Yiyue Sun; Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
  • Hao Fei; Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
  • Qinghong Xue; China Institute of Veterinary Drug Control, Beijing 100081, China.
  • Youxin Zhou; Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China.
  • Hui Wang; Department of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and
  • Peiyan Zheng; Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, The First Affiliated Hosp
  • Hao Chen; Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, The First Affiliated Hosp
  • Feng Cai Zhu; NHC Key laboratory of Enteric Pathogenic Microbiology (Jiangsu Provincial Center for Disease Control
  • Daxin Peng; Colledge of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China.
  • Jun Han; MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
  • Jiwan Qiu; Qyuns Therapeutics, Taizhou, Jiangsu 225316, China.
  • Hongwei Ma; Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
Preprint em En | PREPRINT-BIORXIV | ID: ppbiorxiv-453011
ABSTRACT
Antibody-antigen (Ab-Ag) interactions are canonically described by a model which exclusively accommodates non-interaction (0) or reproducible-interaction (RI) states, yet this model is inadequate to explain often-encountered non-reproducible signals. Here, by monitoring diverse experimental systems and confirmed COVID-19 clinical sera using a peptide microarray, we observed that non-specific interactions (NSI) comprise a substantial proportion of non-reproducible antibody-based results. This enabled our discovery and capacity to reliably identify non-reproducible Ab-Ag interactions (NRI), as well as our development of a powerful explanatory model ("0-RI-NRI-Hook four-state model") that is [mAb]-dependent, regardless of specificity, which ultimately shows that both NSI and NRI are not predictable yet certain-to-happen. In experiments using seven FDA-approved mAb drugs, we demonstrated the use of NSI counts in predicting epitope type. Beyond challenging the centrality of Ab-Ag interaction specificity data in serology and immunology, our discoveries also facilitated the rapid development of a serological test with uniquely informative COVID-19 diagnosis performance.
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Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-BIORXIV Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Preprint
Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-BIORXIV Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Preprint