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AUG-3387, a Human-Derived Monoclonal Antibody Neutralizes SARS-CoV-2 Variants and Reduces Viral Load from Therapeutic Treatment of Hamsters In Vivo
Christopher J Emig; Marco A Mena; Steven J Henry; Adela Vitug; Christian John Ventura; Douglas Fox; Xammy Huu Nguyenla; Haiyue Xu; Chaeho Moon; Sawittree Sahakijjpijarn; Philip J Kuehl; David Revelli; Zhengrong Cui; Robert O Williams III; Dale J Christensen.
Afiliação
  • Christopher J Emig; Augmenta Bioworks
  • Marco A Mena; Augmenta Bioworks
  • Steven J Henry; Augmenta Bioworks
  • Adela Vitug; Augmenta Bioworks
  • Christian John Ventura; Augmenta Bioworks
  • Douglas Fox; School of Public Health, Division of Infectious Diseases and Vaccinology, University of California, Berkeley, 1951 Oxford Street, Berkeley, CA 94720
  • Xammy Huu Nguyenla; Department of Molecular and Cellular Biology,University of California, Berkeley, 1951 Oxford Street, Berkeley, CA 94720
  • Haiyue Xu; 4Molecular Pharmaceutics and Drug Delivery Division, College of Pharmacy, The University of Texas at Austin, 2409 University Avenue, Austin, TX, 78712
  • Chaeho Moon; Molecular Pharmaceutics and Drug Delivery Division, College of Pharmacy, The University of Texas at Austin, 2409 University Avenue, Austin, TX, 78712
  • Sawittree Sahakijjpijarn; TFF Pharmaceuticals
  • Philip J Kuehl; Lovelace Biomedical Research Institute, Albuquerque, NM 87108, USA
  • David Revelli; Lovelace Biomedical Research Institute, Albuquerque, NM 87108, USA
  • Zhengrong Cui; The University of Texas at Austin
  • Robert O Williams III; University of Texas at Austin
  • Dale J Christensen; TFF Pharmaceuticals
Preprint em En | PREPRINT-BIORXIV | ID: ppbiorxiv-464150
ABSTRACT
Infections from the SARS-CoV-2 virus have killed over 4.6 million people since it began spreading through human populations in late 2019. In order to develop a therapeutic or prophylactic antibody to help mitigate the effects of the pandemic, a human monoclonal antibody (mAb) that binds to the SARS-CoV-2 spike protein was isolated from a convalescent patient following recovery from COVID-19 disease. This mAb, designated AUG-3387, demonstrates a high affinity for the spike protein of the original viral strains and all variants tested to date. In vitro pseudovirus neutralization and SARS-CoV-2 neutralization activity has been demonstrated in vitro. In addition, a dry powder formulation has been prepared using a Thin-Film Freezing (TFF) process that exhibited a fine particle fraction (FPF) of 50.95 {+/-} 7.69% and a mass median aerodynamic diameter (MMAD) and geometric standard deviation (GSD) of 3.74 {+/-} 0.73 {micro}m and 2.73 {+/-} 0.20, respectively. The dry powder is suitable for delivery directly to the lungs of infected patients using a dry powder inhaler device. Importantly, AUG-3387, administered as a liquid by intraperitoneal injection or the dry powder formulation delivered intratracheally into Syrian hamsters 24 hours after intranasal SARS-CoV-2 infection, demonstrated a dose-dependent reduction in the lung viral load of the virus. These data suggest that AUG-3387 formulated as a dry powder demonstrates potential to treat COVID-19.
Licença
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Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-BIORXIV Idioma: En Ano de publicação: 2021 Tipo de documento: Preprint
Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-BIORXIV Idioma: En Ano de publicação: 2021 Tipo de documento: Preprint
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