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Rapid metagenomic characterization of a case of imported COVID-19 in Cambodia
Jessica E Manning; Jennifer A Bohl; Sreyngim Lay; Sophana Chea; Sovann Ly; Yi Sengdoeurn; Seng Heng; Chan Vuthy; Katarina Kalantar; Vida Ahyong; Michelle Tan; Jonathan Sheu; Christina M Tato; Joseph DeRisi; Laurence Baril; Philippe Dussart; Veasna Duong; Erik A Karlsson.
Afiliação
  • Jessica E Manning; Laboratory of Malaria and Vector Research, US National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia
  • Jennifer A Bohl; Laboratory of Malaria and Vector Research, US National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia
  • Sreyngim Lay; Laboratory of Malaria and Vector Research, US National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia
  • Sophana Chea; Laboratory of Malaria and Vector Research, US National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia
  • Sovann Ly; Cambodian Center for Disease Control, Ministry of Health, Phnom Penh, Cambodia
  • Yi Sengdoeurn; Cambodian Center for Disease Control, Ministry of Health, Phnom Penh, Cambodia
  • Seng Heng; Cambodian Center for Disease Control, Ministry of Health, Phnom Penh, Cambodia
  • Chan Vuthy; Cambodian Center for Disease Control, Ministry of Health, Phnom Penh, Cambodia
  • Katarina Kalantar; Chan Zuckerberg Initiative, Redwood City, California, USA
  • Vida Ahyong; Chan Zuckerberg Biohub, San Francisco, California, USA
  • Michelle Tan; Chan Zuckerberg Biohub, San Francisco, California, USA
  • Jonathan Sheu; Chan Zuckerberg Biohub, San Francisco, California, USA
  • Christina M Tato; Chan Zuckerberg Biohub, San Francisco, California, USA
  • Joseph DeRisi; UC San Francisco
  • Laurence Baril; Institut Pasteur du Cambodge, Phnom Penh, Cambodia
  • Philippe Dussart; Virology Unit, Insititut Pasteur du Cambodge, Phnom Penh, Cambodia
  • Veasna Duong; Virology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia
  • Erik A Karlsson; Virology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia
Preprint em En | PREPRINT-BIORXIV | ID: ppbiorxiv-968818
ABSTRACT
Rapid production and publication of pathogen genome sequences during emerging disease outbreaks provide crucial public health information. In resource-limited settings, especially near an outbreak epicenter, conventional deep sequencing or bioinformatics are often challenging. Here we successfully used metagenomic next generation sequencing on an iSeq100 Illumina platform paired with an open-source bioinformatics pipeline to quickly characterize Cambodias first case of COVID-2019.
Licença
cc_no
Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-BIORXIV Idioma: En Ano de publicação: 2020 Tipo de documento: Preprint
Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-BIORXIV Idioma: En Ano de publicação: 2020 Tipo de documento: Preprint