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Scalable and Resilient SARS-CoV2 testing in an Academic Centre
Jim Aitken; Karen Ambrose; Sam Barrell; Rupert Beale; Ganka Bineva-Todd; Dhruva Biswas; Richard Byrne; Simon Caidan; Peter Cherepanov; Laura Churchward; Graham Clark; Marg Crawford; Laura Cubitt; Vicky Dearing; Christopher Earl; Amelia Edwards; Chris Ekin; Efthymios Fidanis; Alessandra Gaiba; Steve Gamblin; Sonia Gandhi; Jacki Goldman; Robert Goldstone; Paul R Grant; Maria Greco; Judith Heaney; Steve Hindmarsh; Catherine F Houlihan; Michael Howell; Michael Hubank; Debbie Hughes; Rachel Instrell; Deb Jackson; Mariam Jamal-Hanjani; Ming Jiang; Mark Johnson; Leigh Jones; Nnennaya Kanu; George Kassiotis; Stuart Kirk; Svend Kjaer; Andrew Levett; Lisa Levett; Marcel Levi; Wei-Ting Lu; James I MacRae; John Matthews; Laura McCoy; Catherine Moore; David Moore; Eleni Nastouli; Jerome Nicod; Luke Nightingale; Jessica Olsen; Nicola OReilly; Amar Pabari; Venizelos Papayannopoulos; Namita Patel; Nigel Peat; Marc Pollitt; Peter Ratcliffe; Caetano Reis e Sousa; Annachiara Rosa; Rachel Rosenthal; Chloe Roustan; Andrew Rowan; Gee Yen Shin; Daniel M Snell; Ok-Ryul Song; Moria Spyer; Amy Strange; Charles Swanton; James M A Turner; Melanie Turner; Andreas Wack; Philip A Walker; Sophie Ward; Wai Keong Wong; Joshua Wright; Mary Wu.
Afiliação
  • Jim Aitken; The Francis Crick Institute
  • Karen Ambrose; The Francis Crick Institute
  • Sam Barrell; The Francis Crick Institute
  • Rupert Beale; The Francis Crick Institute
  • Ganka Bineva-Todd; The Francis Crick Institute
  • Dhruva Biswas; University College London
  • Richard Byrne; The Francis Crick Institute
  • Simon Caidan; The Francis Crick Institute
  • Peter Cherepanov; The Francis Crick Institute
  • Laura Churchward; University College London Hospitals, NHS Foundation Trust
  • Graham Clark; The Francis Crick Institute
  • Marg Crawford; The Francis Crick Institute
  • Laura Cubitt; The Francis Crick Institute
  • Vicky Dearing; The Francis Crick Institute
  • Christopher Earl; The Francis Crick Institute
  • Amelia Edwards; The Francis Crick Institute
  • Chris Ekin; Health Services Laboratories
  • Efthymios Fidanis; The Francis Crick Institute
  • Alessandra Gaiba; The Francis Crick Institute
  • Steve Gamblin; The Francis Crick Institute
  • Sonia Gandhi; The Francis Crick Institute
  • Jacki Goldman; The Francis Crick Institute
  • Robert Goldstone; The Francis Crick Institute
  • Paul R Grant; Health Services Laboratories
  • Maria Greco; The Francis Crick Institute
  • Judith Heaney; University College London Hospitals, NHS Foundation Trust
  • Steve Hindmarsh; The Francis Crick Institute
  • Catherine F Houlihan; University College London Hospitals, NHS Foundation Trust
  • Michael Howell; The Francis Crick Institute
  • Michael Hubank; The Institute of Cancer Research
  • Debbie Hughes; The Institute of Cancer Research
  • Rachel Instrell; The Francis Crick Institute
  • Deb Jackson; The Francis Crick Institute
  • Mariam Jamal-Hanjani; University College London
  • Ming Jiang; The Francis Crick Institute
  • Mark Johnson; The Francis Crick Institute
  • Leigh Jones; The Francis Crick Institute
  • Nnennaya Kanu; University College London
  • George Kassiotis; The Francis Crick Institute
  • Stuart Kirk; Health Services Laboratories
  • Svend Kjaer; The Francis Crick Institute
  • Andrew Levett; Health Services Laboratories
  • Lisa Levett; Health Services Laboratories
  • Marcel Levi; University College London Hospitals, NHS Foundation Trust
  • Wei-Ting Lu; The Francis Crick Institute
  • James I MacRae; The Francis Crick Institute
  • John Matthews; Health Services Laboratories
  • Laura McCoy; University College London
  • Catherine Moore; Public Health Wales
  • David Moore; University College London
  • Eleni Nastouli; University College London Hospitals, NHS Foundation Trust
  • Jerome Nicod; The Francis Crick Institute
  • Luke Nightingale; The Francis Crick Institute
  • Jessica Olsen; The Francis Crick Institute
  • Nicola OReilly; The Francis Crick Institute
  • Amar Pabari; Health Services Laboratories
  • Venizelos Papayannopoulos; The Francis Crick Institute
  • Namita Patel; The Francis Crick Institute
  • Nigel Peat; The Francis Crick Institute
  • Marc Pollitt; The Francis Crick Institute
  • Peter Ratcliffe; The Francis Crick Institute
  • Caetano Reis e Sousa; The Francis Crick Institute
  • Annachiara Rosa; The Francis Crick Institute
  • Rachel Rosenthal; The Francis Crick Institute
  • Chloe Roustan; The Francis Crick Institute
  • Andrew Rowan; The Francis Crick Institute
  • Gee Yen Shin; Health Services Laboratories
  • Daniel M Snell; The Francis Crick Institute
  • Ok-Ryul Song; The Francis Crick Institute
  • Moria Spyer; University College London
  • Amy Strange; The Francis Crick Institute
  • Charles Swanton; The Francis Crick Institute
  • James M A Turner; The Francis Crick Institute
  • Melanie Turner; Health Services Laboratories
  • Andreas Wack; The Francis Crick Institute
  • Philip A Walker; The Francis Crick Institute
  • Sophie Ward; The Francis Crick Institute
  • Wai Keong Wong; University College London Hospitals, NHS Foundation Trust
  • Joshua Wright; The Francis Crick Institute
  • Mary Wu; The Francis Crick Institute
Preprint em En | PREPRINT-MEDRXIV | ID: ppmedrxiv-20071373
ABSTRACT
The emergence of the novel coronavirus SARS-CoV-2 has led to a pandemic infecting more than two million people worldwide in less than four months, posing a major threat to healthcare systems. This is compounded by the shortage of available tests causing numerous healthcare workers to unnecessarily self-isolate. We provide a roadmap instructing how a research institute can be repurposed in the midst of this crisis, in collaboration with partner hospitals and an established diagnostic laboratory, harnessing existing expertise in virus handling, robotics, PCR, and data science to derive a rapid, high throughput diagnostic testing pipeline for detecting SARS-CoV-2 in patients with suspected COVID-19. The pipeline is used to detect SARS-CoV-2 from combined nose-throat swabs and endotracheal secretions/ bronchoalveolar lavage fluid. Notably, it relies on a series of in-house buffers for virus inactivation and the extraction of viral RNA, thereby reducing the dependency on commercial suppliers at times of global shortage. We use a commercial RT-PCR assay, from BGI, and results are reported with a bespoke online web application that integrates with the healthcare digital system. This strategy facilitates the remote reporting of thousands of samples a day with a turnaround time of under 24 hours, universally applicable to laboratories worldwide.
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Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-MEDRXIV Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Preprint
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google
Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-MEDRXIV Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Preprint