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Standard operating procedures for SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay
Michael D. Buck; Enzo Z. Poirier; Ana Cardoso; Bruno Frederico; Johnathan Canton; Sam Barrell; Rupert Beale; Richard Byrne; Simon Caidan; Margaret Crawford; Laura Cubitt; Steve Gamblin; Sonia Gandhi; Robert Goldstone; Paul R. Grant; Kiran Gulati; Steve Hindmarsh; Michael Howell; Michael Hubank; Rachael Instrell; Ming Jiang; George Kassiotis; Wei-Ting Lu; James I. MacRae; Iana Martini; Davin Miller; David Moore; Eleni Nastouli; Jerome Nicod; Luke Nightingale; Jessica Olsen; Amin Oomatia; Nicola O'Reilly; Anett Rideg; Ok-Ryul Song; Amy Strange; Charles Swanton; Samra Turajlic; Philip A. Walker; Mary Wu; Caetano Reis e Sousa; - Crick COVID-19 Consortium.
Afiliação
  • Michael D. Buck; The Francis Crick Institute
  • Enzo Z. Poirier; The Francis Crick Institute
  • Ana Cardoso; The Francis Crick Institute
  • Bruno Frederico; The Francis Crick Institute
  • Johnathan Canton; The Francis Crick Institute
  • Sam Barrell; The Francis Crick Institute
  • Rupert Beale; The Francis Crick Institute; University College London
  • Richard Byrne; The Francis Crick Institute
  • Simon Caidan; The Francis Crick Institute
  • Margaret Crawford; The Francis Crick Institute
  • Laura Cubitt; The Francis Crick Institute
  • Steve Gamblin; The Francis Crick Institute
  • Sonia Gandhi; The Francis Crick Institute; University College London; University College London Hospitals, NHS Foundation Trust
  • Robert Goldstone; The Francis Crick Institute
  • Paul R. Grant; Health Service Laboratories
  • Kiran Gulati; New England Biolabs
  • Steve Hindmarsh; The Francis Crick Institute
  • Michael Howell; The Francis Crick Institute
  • Michael Hubank; The Royal Marsden Hospital; The Institute of Cancer Research
  • Rachael Instrell; The Francis Crick Institute
  • Ming Jiang; The Francis Crick Institute
  • George Kassiotis; The Francis Crick Institute
  • Wei-Ting Lu; The Francis Crick Institute
  • James I. MacRae; The Francis Crick Institute
  • Iana Martini; The Royal Free Hospital
  • Davin Miller; New England Biolabs
  • David Moore; University College London; University College London Hospitals, NHS Foundation Trust
  • Eleni Nastouli; University College London Hospitals, NHS Foundation Trust; University College London GOS Institute of Child Health
  • Jerome Nicod; The Francis Crick Institute
  • Luke Nightingale; The Francis Crick Institute
  • Jessica Olsen; The Francis Crick Institute
  • Amin Oomatia; The Royal Free Hospital
  • Nicola O'Reilly; The Francis Crick Institute
  • Anett Rideg; The Francis Crick Institute
  • Ok-Ryul Song; The Francis Crick Institute
  • Amy Strange; The Francis Crick Institute
  • Charles Swanton; The Francis Crick Institute; University College London; University College London Hospitals, NHS Foundation Trust
  • Samra Turajlic; The Francis Crick Institute; The Royal Marsden Hospital
  • Philip A. Walker; The Francis Crick Institute
  • Mary Wu; The Francis Crick Institute
  • Caetano Reis e Sousa; The Francis Crick Institute
  • - Crick COVID-19 Consortium;
Preprint em En | PREPRINT-MEDRXIV | ID: ppmedrxiv-20142430
ABSTRACT
The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority of patient samples is assessed for viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals and an accredited clinical diagnostic laboratory, established a diagnostic testing pipeline that has reported on more than 40,000 RT-qPCR results since its commencement at the beginning of April 2020. However, due to ongoing demand and competition for critical resources, alternative testing strategies were sought. In this work, we present a clinically-validated standard operating procedure (SOP) for high-throughput SARS-CoV-2 detection by RT-LAMP in 25 minutes that is robust, reliable, repeatable, sensitive, specific, and inexpensive.
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Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-MEDRXIV Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Preprint
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google
Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-MEDRXIV Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Preprint