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SARS-CoV-2 Detection in Sewage in Santiago, Chile - Preliminary results.
Manuel Ampuero; Santiago Valenzuela; Fernando Valiente-Echeverria; Ricardo Soto-Rifo; Gonzalo P. Barriga; Jonas Chnaiderman; Cecilia Rojas; Sergio Guajardo-Leiva; Beatriz Diez; Aldo Gaggero.
Afiliação
  • Manuel Ampuero; Laboratory of Environmental Virology, Virology Program, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile
  • Santiago Valenzuela; Laboratory of Environmental Virology, Virology Program, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile
  • Fernando Valiente-Echeverria; Laboratory of Molecular and Cellular Virology, Virology Program, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile.
  • Ricardo Soto-Rifo; Laboratory of Molecular and Cellular Virology, Virology Program, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile
  • Gonzalo P. Barriga; Laboratory of Emerging Viruses, Virology Program, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile
  • Jonas Chnaiderman; Laboratory of Environmental Virology, Virology Program, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile
  • Cecilia Rojas; Laboratory of Environmental Virology, Virology Program, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile
  • Sergio Guajardo-Leiva; Department of Molecular Genetics and Microbiology, Pontifical Catholic University of Chile
  • Beatriz Diez; Department of Molecular Genetics and Microbiology, Pontifical Catholic University of Chile. Center for Climate and Resilience Research (CR)2, Santiago, Chile.
  • Aldo Gaggero; Laboratory of Environmental Virology, Virology Program, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile
Preprint em En | PREPRINT-MEDRXIV | ID: ppmedrxiv-20145177
ABSTRACT
The detection of viruses in sewage is a method of environmental surveillance, which allows evaluating the circulation of different viruses in a community. This study presents the first results of sewage surveillance to detect the circulation of SARS-CoV-2 virus in Santiago, Chile. Using ultracentrifugation associated with RT-qPCR, we detected SARS-CoV-2 in untreated and treated wastewater samples obtained two treatment plants, which together process around 85% of the wastewater from the city. This is the first report of detection of SARS-CoV-2 in sewage in Chile and indicates that wastewater surveillance could be a sensitive tool useful as a predictive marker of the circulation of the virus in a population and therefore, be used as an early warning tool.
Licença
cc_no
Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-MEDRXIV Tipo de estudo: Diagnostic_studies / Experimental_studies / Prognostic_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Preprint
Texto completo: 1 Coleções: 09-preprints Base de dados: PREPRINT-MEDRXIV Tipo de estudo: Diagnostic_studies / Experimental_studies / Prognostic_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Preprint