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High titers of infectious SARS-CoV-2 in COVID-19 corpses
Hisako Saitoh; Yuko Tagawa Sakai; Sayaka Nagasawa; Suguru Torimitsu; Kazumi Kubota; Yuichiro Hirata; Kiyoko Horimoto Iwatsuki; Ayumi Motomura; Namiko Ishii; Keisuke Okaba; Kie Horioka; Hiroyuki Abe; Masako Ikemura; Hirofumi Rokutan; Munetoshi Hinata; Akiko Iwasaki; Yoichi Yasunaga; Makoto Nakajima; Rutsuko Yamaguchi; Shigeki Tsuneya; Kei Kira; Susumu Kobayashi; Go Inokuchi; Fumiko Chiba; Yumi Hoshioka; Aika Mori; Isao Yamamoto; Kimiko Nakagawa; Harutaka Katano; Shun Iida; Tadaki Suzuki; Shinji Akitomi; Iwao Hasegawa; Tetsuo Ushiku; Daisuke Yajima; Hirotaro Iwase; Yohsuke Makino; Yoshihiro Kawaoka.
Afiliação
  • Hisako Saitoh; Department of Legal Medicine, Graduate School of Medicine, Chiba University
  • Yuko Tagawa Sakai; Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, The University of Tokyo
  • Sayaka Nagasawa; Department of Legal Medicine, Graduate School of Medicine, Chiba University
  • Suguru Torimitsu; Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo
  • Kazumi Kubota; Department of Healthcare Information Management, The University of Tokyo Hospital
  • Yuichiro Hirata; Department of Pathology, National Institute of Infectious Diseases
  • Kiyoko Horimoto Iwatsuki; Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, The University of Tokyo
  • Ayumi Motomura; Department of Legal Medicine, International University of Health and Welfare
  • Namiko Ishii; Department of Legal Medicine, International University of Health and Welfare
  • Keisuke Okaba; Department of Legal Medicine, International University of Health and Welfare
  • Kie Horioka; Department of Legal Medicine, International University of Health and Welfare
  • Hiroyuki Abe; Department of Pathology, Graduate School of Medicine, The University of Tokyo
  • Masako Ikemura; Department of Pathology, Graduate School of Medicine, The University of Tokyo
  • Hirofumi Rokutan; Department of Pathology, Graduate School of Medicine, The University of Tokyo
  • Munetoshi Hinata; Department of Pathology, Graduate School of Medicine, The University of Tokyo
  • Akiko Iwasaki; Department of Pathology, Graduate School of Medicine, The University of Tokyo
  • Yoichi Yasunaga; Department of Pathology, Graduate School of Medicine, The University of Tokyo
  • Makoto Nakajima; Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo
  • Rutsuko Yamaguchi; Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo
  • Shigeki Tsuneya; Department of Legal Medicine, Graduate School of Medicine, Chiba University
  • Kei Kira; Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo
  • Susumu Kobayashi; Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo
  • Go Inokuchi; Department of Legal Medicine, Graduate School of Medicine, Chiba University
  • Fumiko Chiba; Department of Legal Medicine, Graduate School of Medicine, Chiba University
  • Yumi Hoshioka; Department of Legal Medicine, Graduate School of Medicine, Chiba University
  • Aika Mori; Department of Legal Medicine, Graduate School of Medicine, Chiba University
  • Isao Yamamoto; Department of Forensic Medicine, Kanagawa Dental University
  • Kimiko Nakagawa; Department of Forensic Medicine, Kanagawa Dental University
  • Harutaka Katano; Department of Pathology, National Institute of Infectious Diseases
  • Shun Iida; Department of Pathology, National Institute of Infectious Diseases
  • Tadaki Suzuki; Department of Pathology, National Institute of Infectious Diseases
  • Shinji Akitomi; Japan Medical Association Research Institute
  • Iwao Hasegawa; Department of Forensic Medicine, Kanagawa Dental University
  • Tetsuo Ushiku; Department of Pathology, Graduate School of Medicine, The University of Tokyo
  • Daisuke Yajima; Department of Legal Medicine, International University of Health and Welfare
  • Hirotaro Iwase; Department of Legal Medicine, Graduate School of Medicine, Chiba University
  • Yohsuke Makino; Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo
  • Yoshihiro Kawaoka; University of Wisconsin-Madison
Preprint em Inglês | medRxiv | ID: ppmedrxiv-22280868
ABSTRACT
BackgroundThe prolonged presence of infectious severe acute respiratory syndrome coronavirus (SARS-CoV-2) in deceased coronavirus disease 2019 (COVID-19) patients has been reported. However, infectious virus titers have not been determined. Such information is important for public health, death investigation, and handling corpses. AimThe aim of this study was to assess the level of SARS-CoV-2 infectivity in COVID-19 corpses. MethodsWe collected 11 nasopharyngeal swabs and 19 lung tissue specimens from 11 autopsy cases with COVID-19 in 2021. We then investigated the viral genomic copy number by real-time reverse transcription-polymerase chain reaction and infectious titers by cell culture and virus isolation. ResultsInfectious virus was present in 6 of 11 (55%) cases, 4 of 11 (36%) nasopharyngeal swabs, and 9 of 19 (47%) lung specimens. The virus titers ranged from 6.00E + 01 plaque-forming units (PFU)/mL to 2.09E + 06 PFU/g. In all cases in which an infectious virus was found, the time from death to discovery was within 1 day and the longest postmortem interval was 13 days. ConclusionCOVID-19 corpses may have high titers of infectious virus after a long postmortem interval (up to 13 days). Therefore, appropriate infection control measures must be taken when handling corpses.
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Texto completo: Disponível Coleções: Preprints Base de dados: medRxiv Tipo de estudo: Estudo observacional Idioma: Inglês Ano de publicação: 2022 Tipo de documento: Preprint
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google
Texto completo: Disponível Coleções: Preprints Base de dados: medRxiv Tipo de estudo: Estudo observacional Idioma: Inglês Ano de publicação: 2022 Tipo de documento: Preprint