The interdomain region of dengue NS5 protein interacts with NS3 and host proteins.
Artigo
em Inglês
| WHO IRIS
| ID: who-163711
Biblioteca responsável:
CH1.1
ABSTRACT
Although dengue virus genome replication occurs in the cytoplasm of infected cells, it has been shown that the NS5 protein (RNA-dependent RNA polymerase) is hyperphosphorylated at a late stage in infection and localized to the cell nucleus. A 37 amino acid sequence of NS5 (residues 369-405)was shown to contain a functional unclear localization signal (NLS) that interacted with the cellular nuclear transport factor, importin α/β heterodimer. Further studies using the yeast two-hybrid system revealed that the NS5 region (residues 320-368) immediately adjacent to the NLS contained an importin β-binding site that abuts or overlaps the binding site for the NS3 protein (protease/helicase). The importin β-binding site has also been shown to be a functional NLS (bNLS). Intriguingly, when both bNLS and NLS (residues 320-405) were present,the fused β -galactosidase protein did not accumulate in the nucleus. Here we provide a review of our studies on the NS5 interdomain region and compare it to other members of the Flavivirus genus in order to highlight the importance of this region as a possible target for developing broad-acting antiviral agent against dengue and other mechanistically-related viruses.
Texto completo:
Disponível
Coleções:
Bases de dados de organismos internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Dengue
/
Doenças Negligenciadas
Base de dados:
WHO IRIS
Assunto principal:
Vírus de RNA
/
Proteínas
/
Sinais de Localização Nuclear
/
Dengue
Idioma:
Inglês
Ano de publicação:
2002
Tipo de documento:
Artigo