Establishment of quality evaluation methods for pieces and standard decoction of honey-fried Descurainiae Semen / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 884-889, 2020.
Article
em Zh
| WPRIM
| ID: wpr-1008514
Biblioteca responsável:
WPRO
ABSTRACT
To establish a content determination method for quality control of the pieces and standard decoction of honey-fried Descurainiae Semen. Standard decoction of honey-fried Descurainiae Semen was prepared with standardized process, and high performance liquid chromatography coupled with diode-array detector(HPLC-DAD) was used to detect its characteristic fingerprint and determine the content of quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside. In addition, the transfer rate, dry extract rate and pH value were calculated. The results showed that the established method had a high accuracy. The content of quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside in 13 batches of standard decoction was 0.03-0.12 mg·mL~(-1); the transfer rate was 13.4%-23.1%; the rate of extracts was 1.9%-5.5%, and the pH was between 5.4-5.9. The similarity coefficients were all greater than 0.85, indicating good homogeneity for the different batches of decoction. There were 7 common peaks in the characteristic chromatogram, one of which was quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside. In this paper, the established content determination and quality evaluation method for Descurainiae Semen pieces and decoction was simple, rapid and reproducible, providing reference for the quality control of honey-fried Descurainiae Semen pieces, standard decoction and its preparations.
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Base de dados:
WPRIM
Assunto principal:
Controle de Qualidade
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Quercetina
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Medicamentos de Ervas Chinesas
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Cromatografia Líquida de Alta Pressão
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Brassicaceae
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Glucosídeos
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Mel
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2020
Tipo de documento:
Article