Experimental study on effect of angelica injection to cognitive impairment after rat chronic cerebral ischemia / 中华神经医学杂志

ABSTRACT

Objective To investigate the effect of angelica injection to vascular cognitive impairment,we observed the change of vascular cognitive function,oxidative damage and eholinergic system after chronic cerebral ischemia. Methods 50 rats were divided into sham operation (n=16), saline group(n=18) and angelica treatment group by random number table. The latter two grous chronic cerebral ischemia models were made by permanent bilateral occlusion of common carotid arteries. After 4 weeks' ischemia and 4 weeks' treatment, Spatial learning and memory abilities were evaluated by Morris water maze, the cortex were detected for oxidative production and acetylcholine esterase. Results After chronic cerebral ischemia, the spatial learning and memory abilities were destroyed, the latency of angelica treatment group in the fouth and fifth day'Morris water maze obviously shortened (27.67±1.34、22.53±1.27), and had significant difference with normal saline group(36.03±1.94、31.05±1.89). In spatial exploring testing the frequency of crossing platform of angelica treatment group increased(1.25±0.19) and the residence time prolonged(21.99±1.24). The contents of acetylcholine esterase increased after chronic cerebral ischemia (0.33±0.04)and the contents of angelica treatment group (0.29±0.05)descended, but had no significant difference. The oxidative production of normal saline group increased, the contents of MDA increased(3.37±0.22), the vigor of SOD(38.24±1.53)and the ability of inhibiting hydroxy radical (18.53±1.18) and total antioxidant (0.96±0.06)decreased. Angelica injection can inhibit oxidative production (MDA 2.38±0.18,the vigor of SOD 60.13±3.92,the ability of inhibiting hydroxy radical 30.76±2.47 ,the ability of total antioxidant 1.60±0.15),and had significant difference with normal saline group. Conclusion Angelica injection could improve cognitive function by inhibiting oxidative stress.