Effect of ACNU on scores of modified neurological severity scale in rat normal brain tissues / 中华神经医学杂志

ABSTRACT

[Objective] To study the effect of ACNU,a kind of antineoplastic,on scores of modified neurological severity scale (mNSS) when it with different concentrations contacted to the local cerebral tissues of rats.[Methods]Forty-five SD rats were randomly divided into 5 groups,including Group A,B,C and D (n=10) and Group E (n=5).After being anesthetized,the parietal of the rats was opened to exposure the left cerebral hemisphere,and then the gelfoam (specification as 2 mm x2 mm x2 mam) dipped with the different doses (2,1,0.5,0.25 and 0 mg/kg) of ACNU solution were respectively applied on the surface of the rat hemisphere in Group A,B,C and D and Group E.The wound was then surgically closed.The mNSS scores in every group were respectively evaluated at 6 h,and 1,3,7,10 and 14 d after the administration of ACNU.[Results] The mNSS scores showed significant difference between each 2 groups (P＜0.05);the trends ofmNSS scores were also obviously different at different times after the administration of ACNU (P＜0.05);the changes were much stable in Group D and E;at the same time point,the mNSS scores in Group A,B and C were significantly higher than those in Group D and E (P＜0.05);the mNSS scores were increased at first and then decreased following the prolongation of administration time.[Conclusion] The 0.25 mg/kg of ACNU or lower doses of that could be considered as safe doses for applying to the normal cerebral tissue ofrats.