Role of protein kinase C in the mechanism of central sensitization of migraine / 中华神经医学杂志

ABSTRACT

Objective To explore the role of protein kinase C (PKC) in the mechanism of central sensitization of migraine.Methods Sixty healthy adult male SD rats,weighting from 200 to 250 g,were randomly divided into five groups:normal group,sham-operated group,migraine model group,chloroform treatment group and H-7 (the inhibitor of PKC) treatment group (n=12).Dural blood flow monitor was performed by laser Doppler blood flow imager and extracellular discharge frequency in the spinal trigeminal nucleus was observed by multi-conductive polygraph; the dural blood flow and discharge frequency changes were analyzed and compared.Results Two hours after the success of model making,the dural blood flow in the migraine model group increased obviously as compared with that in the sham-operated group (P＜0.05); as compared with that in the migraine model group,the dural blood flow in the H-7 treatment group decreased obviously (P＜0.05); as compared with sham operation group,blood flow decreased obviously in H-7 group (P＜0.05).Extracellular discharge frequency in the spinal trigeminal nucleus increased 2 h after the success of model making; 2 hours after model making,the extracellular discharge frequency was (323.82±11.00) ％ of baseline level; as compared with that in the migraine model group,discharge frequency in the H-7 treatment group decreased obviously (P＜0.05); as compared with that in the sham-operated group,discharge frequency in the H-7 treatment group had no obvious changes (P＞0.05).Conclusion PKC may play an important role in the mechanism of central sensitization of migraine.