Your browser doesn't support javascript.
loading
The Cytotoxicity of Kahweol in HT-29 Human Colorectal Cancer Cells Is Mediated by Apoptosis and Suppression of Heat Shock Protein 70 Expression
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-104383
Biblioteca responsável: WPRO
ABSTRACT
Although coffee is known to have antioxidant, anti-inflammatory, and antitumor properties, there have been few reports about the effect and mechanism of coffee compounds in colorectal cancer. Heat shock proteins (HSPs) are molecular chaperones that prevent cell death. Their expression is significantly elevated in many tumors and is accompanied by increased cell proliferation, metastasis and poor response to chemotherapy. In this study, we investigated the cytotoxicity of four bioactive compounds in coffee, namely, caffeine, caffeic acid, chlorogenic acid, and kahweol, in HT-29 human colon adenocarcinoma cells. Only kahweol showed significant cytotoxicity. Specifically, kahweol increased the expression of caspase-3, a pro-apoptotic factor, and decreased the expression of anti-apoptotic factors, such as Bcl-2 and phosphorylated Akt. In addition, kahweol significantly attenuated the expression of HSP70. Inhibition of HSP70 activity with triptolide increased kahweol-induced cytotoxicity. In contrast, overexpression of HSP70 significantly reduced kahweol-induced cell death. Taken together, these results demonstrate that kahweol inhibits colorectal tumor cell growth by promoting apoptosis and suppressing HSP70 expression.
Assuntos

Texto completo: Disponível Contexto em Saúde: ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis Problema de saúde: Neoplasias Colorretais Base de dados: WPRIM (Pacífico Ocidental) Assunto principal: Cafeína / Neoplasias Colorretais / Adenocarcinoma / Ácido Clorogênico / Morte Celular / Apoptose / Café / Colo / Chaperonas Moleculares / Proteínas de Choque Térmico HSP70 Limite: Humanos Idioma: Inglês Revista: Biomolecules & Therapeutics Ano de publicação: 2015 Tipo de documento: Artigo
Texto completo: Disponível Contexto em Saúde: ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis Problema de saúde: Neoplasias Colorretais Base de dados: WPRIM (Pacífico Ocidental) Assunto principal: Cafeína / Neoplasias Colorretais / Adenocarcinoma / Ácido Clorogênico / Morte Celular / Apoptose / Café / Colo / Chaperonas Moleculares / Proteínas de Choque Térmico HSP70 Limite: Humanos Idioma: Inglês Revista: Biomolecules & Therapeutics Ano de publicação: 2015 Tipo de documento: Artigo
...