Codelivery of IL-7 Augments Multigenic HCV DNA Vaccine-induced Antibody as well as Broad T Cell Responses in Cynomolgus Monkeys
Immune Network
; : 198-205, 2010.
Article
em En
| WPRIM
| ID: wpr-10995
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: A crucial limitation of DNA vaccines is its weak immunogenicity, especially in terms of eliciting antibody responses in non-human primates or humans; therefore, it is essential to enhance immune responses to vaccination for the development of successful DNA vaccines for humans. METHODS: Here, we approached this issue by evaluating interleukin-7 (IL-7) as a genetic adjuvant in cynomolgus monkeys immunized with multigenic HCV DNA vaccine. RESULTS: Codelivery of human IL-7 (hIL-7)-encoding DNA appeared to increase DNA vaccine-induced antibody responses specific for HCV E2 protein, which plays a critical role in protecting from HCV infection. HCV-specific T cell responses were also significantly enhanced by codelivery of hIL-7 DNA. Interestingly, the augmentation of T cell responses by codelivery of hIL-7 DNA was shown to be due to the enhancement of both the breadth and magnitude of immune responses against dominant and subdominant epitopes. CONCLUSION: Taken together, these findings suggest that the hIL-7-expressing plasmid serves as a promising vaccine adjuvant capable of eliciting enhanced vaccine-induced antibody and broad T cell responses.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Plasmídeos
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Primatas
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DNA
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Interleucina-7
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Vacinação
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Vacinas de DNA
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Macaca fascicularis
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Formação de Anticorpos
Limite:
Humans
Idioma:
En
Revista:
Immune Network
Ano de publicação:
2010
Tipo de documento:
Article