Occurrence of a PCR-Positive but Culture-Negative Case for vanB Vancomycin-Resistant Enterococci in Stool Surveillance
Laboratory Medicine Online
; : 264-268, 2013.
Article
em Ko
| WPRIM
| ID: wpr-114466
Biblioteca responsável:
WPRO
ABSTRACT
We present here occurence of PCR-positive but culture-negative for vanB vancomycin-resistant enterococci (VRE) from an enrichment broth of a stool surveillance culture in a patient suffering from Parkinson's disease, who was transferred from a long-term care facility because of aspiration pneumonia. He developed VRE bacteriuria at the hospital day 42. vanA and vanB genes were detected from 6 microg/mL vancomycin-containing BBL Enterococcosel broth (BD), of which color changed to black after overnight incubation, by both Seeplex VRE detection (Seegene, Seoul, Korea) and Anyplex VanR real-time PCR (Seegene). Subculture of an aliquot of the blackened broth on blood agar plate produced only vanA VRE. All of the four subsequent consecutive surveillance cultures for 1 month until discharge at hospital day 75 resulted in PCR-positive but culture-negative for vanB VRE from the enrichment broths. Therefore, the presence of a non-enterococcal intrinsic reservoir bearing vanB is more likely than low burden of vanB VRE. Considering the rare occurrence of vanB VRE in Korea, vanB-positive PCR results from the enrichment broth requires confirmation by microbiological studies.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Doença de Parkinson
/
Pneumonia Aspirativa
/
Estresse Psicológico
/
Bacteriúria
/
Ursidae
/
Vancomicina
/
Reação em Cadeia da Polimerase
/
Enterococcus
/
Assistência de Longa Duração
/
Ágar
Tipo de estudo:
Screening_studies
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
Ko
Revista:
Laboratory Medicine Online
Ano de publicação:
2013
Tipo de documento:
Article