Phylogenetic Analysis of the 56-kDa Type-Specific Protein Genes of Orientia tsutsugamushi in Central Korea
Journal of Korean Medical Science
; : 1315-1319, 2012.
Article
em En
| WPRIM
| ID: wpr-128889
Biblioteca responsável:
WPRO
ABSTRACT
There are several antigenic variants of Orientia tsutsugamushi. The 56-kDa type-specific antigen (TSA) is responsible for the antigenic variation. Nucleotide sequences of the 56-kDa TSA obtained from 44 eschar samples of Korean scrub typhus patients and from 40 representative strains retrieved from the GenBank database were analyzed phylogenetically. Clinical patient data were assessed based on the genotyping results. Of the 44 nucleotide sequences, 32 (72.7%) clustered with the Boryong genotype, which is the major genotype in Korea. Eleven nucleotide sequences (25%) clustered with the Kawasaki genotype, not identified in Korea until 2010. One nucleotide sequence was consistent with the Karp genotype. The clinical course of the patients infected with each genotype showed no differences. Diagnostic performance of the immunofluorescence assay (IFA) using the 56-kDa TSA from Gilliam, Karp and Boryong as test antigens were not different for the Boryong and Kawasaki genotypes. Although Boryong is still the predominant genotype, the results suggest that Kawasaki genotype is quite prevalent in Chungbuk province of Korea.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Filogenia
/
Orientia tsutsugamushi
/
Proteínas de Bactérias
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DNA Bacteriano
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Tifo por Ácaros
/
Sequência de Bases
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Fases de Leitura Aberta
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Análise de Sequência de DNA
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Bases de Dados Genéticas
/
República da Coreia
Tipo de estudo:
Prognostic_studies
Limite:
Aged
/
Female
/
Humans
/
Male
País/Região como assunto:
Asia
Idioma:
En
Revista:
Journal of Korean Medical Science
Ano de publicação:
2012
Tipo de documento:
Article