Correlation of HLA-G Gene Expression with Various Prognostic Factors in the Tissue of Ovarian Carcinoma / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
; : 2910-2918, 2005.
Article
em Ko
| WPRIM
| ID: wpr-150616
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE: The objective of this study was to investigate the relationship between the expression of mRNA and protein of HLA-G and other clinicopathologic prognostic factors of ovarian cancer. METHODS: 43 patients diagnosed with ovarian cancer from 1997 to 2005 and 5 patients with normal ovarian tissue (controls) were enrolled in this prospective study. The patient group all went through baseline studies with staging laparotomy and adjuvant chemotherapy. Quantitative real-time RT PCR and Western blot analysis were used in detecting the expression of mRNA and protein of HLA-G. RESULTS: The mRNA expression of HLA-G (2-delta delta Ct value) in cancer group were 1.21 (0-9), and 0.01 (0-0.02) in control group, which was statistically significant (P=0.005). The expressed protein levels did not show any difference between the cancer and control groups. There also was a significant relationship between the serum CA 125 at the time of diagnosis and the HLA-G protein levels (P=0.02). But the relationship between other clinicopathologic prognostic factors and the HLA-G protein levels were not statistically significant. CONCLUSION: Our results showed that HLA-G mRNA expression level was much higher in ovarian cancer patient group than in those of control group. Therefore HLA-G maybe play an important role in carcinogenesis of ovarian cancer. Although the protein level of HLA-G had low significance with other prognostic factors, serum CA 125 showed a statistically significant relation with protein levels of HLA-G. Further studies based on the correlation between HLA-G and survival rate are needed to support HLA-G as a prognostic factor of ovarian cancer.
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Base de dados:
WPRIM
Assunto principal:
Neoplasias Ovarianas
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RNA Mensageiro
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Expressão Gênica
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Western Blotting
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Reação em Cadeia da Polimerase
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Taxa de Sobrevida
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Estudos Prospectivos
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Quimioterapia Adjuvante
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Diagnóstico
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Antígenos HLA-G
Tipo de estudo:
Diagnostic_studies
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Observational_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
Ko
Revista:
Korean Journal of Obstetrics and Gynecology
Ano de publicação:
2005
Tipo de documento:
Article