Effect of Exercise on micro-Opioid Receptor Expression in the Rostral Ventromedial Medulla in Neuropathic Pain Rat Model
Annals of Rehabilitation Medicine
; : 331-339, 2015.
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| ID: wpr-153689
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ABSTRACT
OBJECTIVE: To investigate the effects of aerobic exercise on neuropathic pain and verify whether regular treadmill exercise alters opioid receptor expression in the rostral ventral medulla (RVM) in a neuropathic pain rat model. METHODS: Thirty-two male Sprague-Dawley rats were used in the study. All rats were divided into 3 groups, i.e., group A, sham group (n=10); group B, chronic constriction injury (CCI) group (n=11); and group C, CCI+exercise group (n=11). Regular treadmill exercise was performed for 30 minutes a day, 5 days a week, for 4 weeks at the speed of 8 m/min for 5 minutes, 11 m/min for 5 minutes, and 22 m/min for 20 minutes. Withdrawal threshold and withdrawal latency were measured before and after the regular exercise program. Immunohistochemistry and Western blots analyses were performed using antibodies against micro-opioid receptor (MOR). RESULTS: Body weight of group C was the lowest among all groups. Withdrawal thresholds and withdrawal latencies were increased with time in groups B and C. There were significant differences of withdrawal thresholds between group B and group C at 1st, 2nd, 3rd, and 4th weeks after exercise. There were significant differences of withdrawal latencies between group B and group C at 3rd and 4th weeks after exercise. MOR expression of group C was significantly decreased, as compared to that of group B in the RVM and spinal cord. CONCLUSION: In neuropathic pain, exercise induced analgesia could be mediated by desensitization of central MOR by endogenous opioids, leading to the shift of RVM circuitry balance to pain inhibition.
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Base de dados:
WPRIM
Assunto principal:
Medula Espinal
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Peso Corporal
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Bulbo
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Imuno-Histoquímica
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Exercício Físico
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Western Blotting
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Ratos Sprague-Dawley
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Receptores Opioides
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Constrição
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Modelos Animais
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Annals of Rehabilitation Medicine
Ano de publicação:
2015
Tipo de documento:
Article