The Therapeutic Efficacy of Tonsil-derived Mesenchymal Stem Cells in Dextran Sulfate Sodium-induced Acute Murine Colitis Model / 대한소화기학회지
The Korean Journal of Gastroenterology
; : 119-128, 2017.
Article
em Ko
| WPRIM
| ID: wpr-155810
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND/AIMS:
Mesenchymal stem cells (MSCs) are multipotent progenitor cells currently under investigation for its efficacy as the treatment for inflammatory bowel disease. In this study, we evaluated the efficacy of tonsil-derived mesenchymal stem cells (T-MSCs) as a novel source of mesenchymal stem cells and traced their localization in a murine model of acute colitis induced by dextran sulfate sodium (DSS).METHODS:
C57BL/6 mice were randomly assigned to the following three groups the normal control group, DSS colitis group (DSS+phosphate buffered saline), and T-MSC group (DSS+T-MSCs, 1×106). The severity of colitis was assessed by determining the severity of symptoms of colitis, colon length, histopathologic grade, and levels of inflammatory cytokines. T-MSCs labeled with PKH26 were traced in vivo.RESULTS:
The T-MSC group, compared with the DSS colitis group, showed a significantly lower disease activity index (11.3±1.5 vs. 8.3±1.9, p=0.015) at sacrifice and less reduction of body weight (-17.1±5.0% vs. -8.1±6.9%, p=0.049). In the T-MSC group, the histologic colitis score was significantly decreased compared with the DSS colitis group (22.6±3.8 vs. 17.0±3.4, p=0.039). IL-6 and IL-1β, the pro-inflammatory cytokines, were also significantly reduced after a treatment with T-MSCs. In vivo tracking revealed no PKH26-labelled T-MSCs in the colonic tissue of mice with acute colitis.CONCLUSIONS:
In the acute colitis model, we demonstrated that the administration of T-MSCs ameliorates inflammatory symptoms and histology. Moreover, the anti-inflammatory activities of T-MSCs were independent of gut homing.Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Células-Tronco
/
Tonsila Palatina
/
Peso Corporal
/
Doenças Inflamatórias Intestinais
/
Citocinas
/
Sulfato de Dextrana
/
Dextranos
/
Interleucina-6
/
Colite
/
Colo
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Ko
Revista:
The Korean Journal of Gastroenterology
Ano de publicação:
2017
Tipo de documento:
Article