Activation of MAPK Is Required for ROS Generation and Exocytosis in HMC-1 Cells Induced by Trichomonas vaginalis-Derived Secretory Products
The Korean Journal of Parasitology
; : 597-603, 2015.
Article
em En
| WPRIM
| ID: wpr-160903
Biblioteca responsável:
WPRO
ABSTRACT
Trichomonas vaginalis is a flagellated protozoan parasite that causes vaginitis and cervicitis in women and asymptomatic urethritis and prostatitis in men. Mast cells have been reported to be predominant in vaginal smears and vaginal walls of patients infected with T. vaginalis. Mitogen-activated protein kinase (MAPK), activated by various stimuli, have been shown to regulate the transcriptional activity of various cytokine genes in mast cells. In this study, we investigated whether MAPK is involved in ROS generation and exocytotic degranulation in HMC-1 cells induced by T. vaginalis-derived secretory products (TvSP). We found that TvSP induces the activation of MAPK and NADPH oxidase in HMC-1 cells. Stimulation with TvSP induced phosphorylation of MAPK and p47phox in HMC-1 cells. Stimulation with TvSP also induced up-regulation of CD63, a marker for exocytosis, along the surfaces of human mast cells. Pretreatment with MAPK inhibitors strongly inhibited TvSP-induced ROS generation and exocytotic degranulation. Finally, our results suggest that TvSP induces intracellular ROS generation and exocytotic degranulation in HMC-1 via MAPK signaling.
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Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Trichomonas vaginalis
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Degranulação Celular
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Linhagem Celular
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Espécies Reativas de Oxigênio
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Proteínas Quinases Ativadas por Mitógeno
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Fatores de Virulência
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Exocitose
/
Mastócitos
Limite:
Humans
Idioma:
En
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2015
Tipo de documento:
Article