Degradation of phagosomes and diurnal changes of lysosomes in rabbit retinal pigment epithelium
Korean Journal of Ophthalmology
; : 82-91, 1996.
Article
em En
| WPRIM
| ID: wpr-169591
Biblioteca responsável:
WPRO
ABSTRACT
Diurnal changes of lysosomes including ultrastructural changes of phagosomes and acid phosphatase reactions in phagosomes, as well as diurnal biochemical changes in cathepsin D activity, were studied in the retinal pigment epithelium (RPE) of the rabbit. The rabbit was maintained on a natural light-dark cycle over seven days in fall and was sacrificed at various times during the day and night. The number of lysosomes or phagosomes in the RPE was the highest at 1.5 hours after exposure to sunlight (8:00 AM), and thereafter decreased with time. Three types of phagosomes were observed and acid phosphatase reactions were different in each type of phagosome; the fresh phagosomes were negative or positive, lamellar bodies positive, and dense bodies partially positive. The biochemical activity of cathepsin D was the highest at 8:00 AM, and this was consistent with the time of peak in phagocytic activity in the RPE. This report shows that phagocytic activity in the RPE occurred in the early stage after exposure to sunlight, and that fresh phagosomes were sequentially degraded to lamellar or dense bodies. Cathepsin D activity also increased, and this was consistent with the phagocytic activity in the RPE.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Epitélio Pigmentado Ocular
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Fosfatase Ácida
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Fagossomos
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Contagem de Células
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Corioide
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Catepsina D
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Ritmo Circadiano
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Lisossomos
Limite:
Animals
Idioma:
En
Revista:
Korean Journal of Ophthalmology
Ano de publicação:
1996
Tipo de documento:
Article