The Early Diagnosis of Herpetic Ke ratitis by Polymerase Chain Reaction
Journal of the Korean Ophthalmological Society
; : 36-42, 2001.
Article
em Ko
| WPRIM
| ID: wpr-170321
Biblioteca responsável:
WPRO
ABSTRACT
We evaluated the efficacy of polymerase chain reaction(PCR)in the early diagnosis of Herpes simplex virus keratitis(HSK). Among 47 patients(47 eyes), 25 patients were clinically herpetic keratitis and the others were other kinds of keratitis clinically. Tear film specimens were taken from 32 eyes and corneal scrapings from 21 eyes with suspicious herpetic keratitis and clinically incongrous with HSK. PCR for tear and corneal scraping was performed with using DNA primer. 67% of corneal scrapings and 19%tear samples of suspicious herpetic keratitis showed PCR positive. Tear samples from keratitis clinically incongruous with HSK were all PCR negative but 40%of corneal scrapings from these patients represented PCR positive. From these results, PCR is a rapid and effective tool for the early diagnosis of herpetic keratitis and especially useful in cases of nonspecific corneal lesion. However, for the accurate diagnosis of HSK, not only PCR results but also patient's history and corneal findings should be well evaluated.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Lágrimas
/
DNA
/
Reação em Cadeia da Polimerase
/
Ceratite Herpética
/
Simplexvirus
/
Diagnóstico Precoce
/
Diagnóstico
/
Ceratite
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Humans
Idioma:
Ko
Revista:
Journal of the Korean Ophthalmological Society
Ano de publicação:
2001
Tipo de documento:
Article