Inhibitory Effect of Rotundarpene on Parkinsonian Neurotoxin 1-Methyl-4-Phenylpyridinium-Induced Apoptotic Cell Death
Journal of the Korean Neurological Association
; : 324-332, 2016.
Article
em Ko
| WPRIM
| ID: wpr-182774
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND:
The extract and hemiterpene glycosides of Ilex Rotunda Thunb exert antioxidant and anti-inflammatory effects. The effect of rotundarpene on apoptosis in neuronal cells caused by the 1-methyl-4-phenylpyridinium (MPP⁺) has not been reported previously.METHODS:
Using differentiated PC12 cells and human neuroblastoma SH-SY5Y cells, we investigated the effect of rotundarpene on MPP⁺-caused apoptosis in relation to the cell death process.RESULTS:
MPP⁺-induced cell death was identified using the MTT and neutral red uptake tests. Apoptosis was induced by eliciting decreases in the cytosolic levels of Bid and Bcl-2 proteins, increases in the cytosolic levels of Bax and p53, disruption of the mitochondrial transmembrane potential, and the release of cytochrome c and the activation of caspase-8, -9, and -3 in differentiated PC12 cells and SH-SY5Y cells. Treatment with rotundarpene reduced the MPP⁺-induced changes in the levels of apoptosis-regulated proteins, formation of reactive oxygen species, depletion and oxidation of glutathione, and cell death in both PC12 and SH-SY5Y cells.CONCLUSIONS:
Rotundarpene may reduce MPP⁺-induced apoptosis in neuronal cells by suppressing the activation of the mitochondria-mediated pathway and the caspase-8 and Bid pathways. Rotundarpene appears to act by inhibiting the production of reactive oxygen species and by the depletion and oxidation of glutathione.Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
1-Metil-4-fenilpiridínio
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Células PC12
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Morte Celular
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Espécies Reativas de Oxigênio
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Apoptose
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Ilex
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Citosol
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Citocromos c
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Caspase 8
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Glutationa
Limite:
Animals
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Humans
Idioma:
Ko
Revista:
Journal of the Korean Neurological Association
Ano de publicação:
2016
Tipo de documento:
Article