Comparison of Detection Methods of Extended-spectrum beta-lactamase Producing Klebsiella pneumoniae and Escherichia coli / 대한임상미생물학회지
Korean Journal of Clinical Microbiology
; : 62-66, 2001.
Artigo
em Coreano
| WPRIM (Pacífico Ocidental)
| ID: wpr-185331
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND:
Accurate detection of extended spectrum beta-lactamase (ESBL) is important because ESBLs producing organisms may appear susceptible to oxyimino-beta-lactams in standard susceptibility tests, but are considered to be clinically resistant to these drugs. Conventional antimicrobial susceptibility test methods do not reliably detect ESBL production. Molecular techniques and NCCLS broth dilution method, which detect ESBL production, may be time consuming, expensive and technically difficult to perform. The purpose of this study is to evaluate the clinical usefulness of NCCLS ESBL phenotypic confirmatory test by disk diffusion method.METHODS:
For 96 Escherichia coli and 49 Klebsiella pneumoniae isolates collected between December 2000 to February 2001, double disk synergy test, NCCLS ESBL screening and phenotypic confirmatory test by disk diffusion test were performed. The ESBL producer was defined as organism showed an increase in the zone diameter of > or = 5 mm for either antimicrobial agent such as cefotaxime and ceftazidime tested in combination with clavulanic acid versus its zone when tested alone.RESULTS:
The sensitivity of NCCLS ESBL phenotypic confirmatory test were as follows cefotaxime/clavulanic acid disk; 100% in K. pneumoniae and 83% in E. coli, and ceftazidime/clavulanic acid disk; 94% in K. pneumoniae and 67% in E. coli, respectively. Among the organisms with positive to NCCLS ESBL phenotypic confirmatory test, the detection rate of antimicrobial agents in double disk synergy test were as follows K. pneumoniae; cefotaxime (84%), aztreonam (74%), and ceftazidime (52%), and E. coli; cefotaxime (44%), ceftazidime (44%), and aztreonam (39%).CONCLUSIONS:
The NCCLS ESBL phenotypic confirmatory test by disk diffusion method is easy, rapid, and sensitive method, suitable for routine use in the clinical laboratory.
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
/
Doenças Negligenciadas
/
ODS3 - Meta 3.3 Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
Problema de saúde:
Meta 3.4: Reduzir as mortes prematuras devido doenças não transmissíveis
/
Doenças Negligenciadas
/
Zoonoses
/
Pneumonía
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pneumonia
/
Beta-Lactamases
/
Aztreonam
/
Cefotaxima
/
Ceftazidima
/
Programas de Rastreamento
/
Ácido Clavulânico
/
Difusão
/
Escherichia
/
Escherichia coli
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Idioma:
Coreano
Revista:
Korean Journal of Clinical Microbiology
Ano de publicação:
2001
Tipo de documento:
Artigo