Anti-HER2/neu Peptide Producing Vector System for Biologic Therapy - Is It Possible to Mass-produce the Peptide?
Yonsei Medical Journal
; : 58-64, 2003.
Article
em En
| WPRIM
| ID: wpr-186280
Biblioteca responsável:
WPRO
ABSTRACT
A humanized monoclonal antibody against HER2 has been using in a clinical setting and has been shown to possess therapeutic properties. A mimetic peptide against HER2 was also reported to bind to the HER2 receptor with some therapeutic potential. Based on a previous report and the sequence of Herceptin, we designed oligonucleotides of anti-HER2 mimetic peptides, named V2 and V3 peptides, in order to develop a peptide- producing vector system for biologic therapy against HER2- overexpressing cancers. We also adopted the sequence of a previously reported mimetic peptide, V1 (Park BW et al. Nat. Biotechnol, 2000, 18 194-198), as a reference peptide. We examined the effects of the V2 and V3 peptides against the HER2-overexpressing cell lines, SK-BR-3 and T6-17. Transient transfection of the construct expressing V1 and V2 inhibited cell proliferation in HER2-overexpressing cell lines by 20 - 30%, but had no effect on the HER2-negative NIH3T3 cells. The proliferation inhibition shown by V2 was slightly better than that shown by V1. Recombinant peptides V2 and V3 were produced on a large scale in an E. coli system, and the V2 peptide showed anti-HER2-specific tumor cell proliferation inhibition of 10% to 30%. Current results suggest that anti-HER2 mimetic peptides, overexpressed by a constitutive promoter or produced in an E. coli system, could specifically inhibit the proliferation of HER2-expressing cancer cells. Further efforts to augment the biologic specificity and efficacy and to develop new technologies for the purification of the peptide from the E coli system are needed.
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Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Oligopeptídeos
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Fragmentos de Peptídeos
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Proteínas Recombinantes
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Transfecção
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Divisão Celular
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Linhagem Celular
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Sequência de Aminoácidos
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Tecnologia Farmacêutica
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Receptor ErbB-2
Limite:
Animals
Idioma:
En
Revista:
Yonsei Medical Journal
Ano de publicação:
2003
Tipo de documento:
Article
País de publicação:
COREA DEL SUR
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COREIA DO SUL
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KR
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SOUTH KOREA