Blocking junctional adhesion molecule C promotes the recovery of cisplatin-induced acute kidney injury
The Korean Journal of Internal Medicine
; : 1053-1061, 2017.
Article
em En
| WPRIM
| ID: wpr-187141
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND/AIMS:
Recent findings have demonstrated the occurrence of neutrophil transendothelial migration in the reverse direction (reverse TEM) and that endothelial junctional adhesion molecule C (JAM-C) is a negative regulator of reverse TEM. In this study, we tested the effects of a JAM-C blocking antibody on the resolution of kidney injuries and inflammation in a mouse model of cisplatin-induced acute kidney injury (AKI).METHODS:
Cisplatin was administered via intraperitoneal injection. A JAM-C blocking antibody or a control immunoglobulin G was administered intraperitoneal at 1.5 mg/kg, with the injection being delayed until day 4 following cisplatin administration to restrict the effect of antibodies on recovery.RESULTS:
After cisplatin injection, serum creatinine and histologic injuries peaked on day 4. Treatment with a JAM-C blocking antibody on days 4 and 5 promoted the functional and histologic recovery of cisplatin-induced AKI on days 5 and 6. Facilitating recovery with a JAM-C blocking antibody correlated with significantly increased circulating intercellular adhesion molecule 1+ Tamm-Horsfall protein+ neutrophils and significantly decreased renal neutrophil infiltration, indicating that facilitating reverse the TEM of neutrophils from the kidney to the peripheral circulation partially mediated the resolution of inflammation and recovery.CONCLUSIONS:
These results demonstrated that reverse TEM is involved in the resolution of neutrophilic inflammation in cisplatin-induced AKI and that JAM-C is an important regulator of this process.Palavras-chave
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Imunoglobulina G
/
Cisplatino
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Creatinina
/
Infiltração de Neutrófilos
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Injúria Renal Aguda
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Migração Transendotelial e Transepitelial
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Moléculas de Adesão Juncional
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Molécula C de Adesão Juncional
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Inflamação
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Injeções Intraperitoneais
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
The Korean Journal of Internal Medicine
Ano de publicação:
2017
Tipo de documento:
Article