Induction of Potent Antigen-specific Cytotoxic T Cell Response by PLGA-nanoparticles Containing Antigen and TLR Agonist
Immune Network
; : 30-33, 2013.
Article
em En
| WPRIM
| ID: wpr-199843
Biblioteca responsável:
WPRO
ABSTRACT
Previously we showed that biodegradable nanoparticles containing poly-IC or CpG oligodeoxynucleotide (ODN) together with ovalbumin (OVA) were efficient at inducing MHC-restricted presentation of OVA peptides in dendritic cells. The CTL-inducing activities of the nanoparticles were examined in the present study. Nanoparticles containing poly-IC or CpG ODN together with OVA were prepared using biodegradable polymer poly(D,L-lactic acid-co-glycolic acid), and then were opsonized with mouse IgG. The nanoparticles were injected into the tail vein of mice, and 7 days later the OVA-specific CTL activities were measured using an in vivo CTL assay. Immunization of mice with the nanoparticles containing poly-IC or CpG ODN together with OVA elicited potent OVA-specific CTL activity compared to those containing OVA only. In accordance with these results, nanoparticles containing poly-IC or CpG ODN together with OVA exerted potent antitumor activity in mice that were subcutaneously implanted with EG7.OVA tumor cells. These results show that encapsulation of poly-IC or CpG ODN together with antigen in biodegradable nanoparticles is an effective approach for the induction of potent antigen-specific CTL responses in vivo.
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Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Óvulo
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Peptídeos
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Ácido Poliglicólico
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Polímeros
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Veias
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Células Dendríticas
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Imunoglobulina G
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Ovalbumina
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Imunização
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Ácido Láctico
Limite:
Animals
Idioma:
En
Revista:
Immune Network
Ano de publicação:
2013
Tipo de documento:
Article