Macrolide Resistance of Mycoplasma pneumoniae and Its Detection Rate by Real-Time PCR in Primary and Tertiary Care Hospitals
Annals of Laboratory Medicine
; : 410-414, 2013.
Article
em En
| WPRIM
| ID: wpr-214992
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND:
This study aimed to evaluate the prevalence of Mycoplasma pneumoniae in primary and tertiary care hospitals and its macrolide resistance rate.METHODS:
Nasopharyngeal swabs were collected from 195 pediatric patients in primary and tertiary care hospitals from October to November 2010. The AccuPower MP real-time PCR kit (Bioneer, Korea) was used for the detection of M. pneumoniae. Direct amplicon sequencing was performed to detect point mutations conferring resistance to macrolides in the 23S rRNA gene.RESULTS:
Among the 195 specimens, 17 (8.7%) were M. pneumoniae positive, and 3 of the strains (17.6%) obtained from these 17 specimens displayed the A2063G mutation in 23S rRNA. Three macrolide-resistant M. pneumoniae isolates were isolated from patients hospitalized at the primary care hospital. The positive rates of M. pneumoniae for the primary and tertiary care hospitals were 12.1% (15/124) and 2.8% (2/71), respectively (P=0.033).CONCLUSIONS:
The positive rate of M. pneumoniae in the primary care hospital was higher than that in the tertiary care hospital. Simultaneous detection of M. pneumoniae and macrolide-resistant mutation genes in the 23S rRNA by real-time PCR is needed for rapid diagnosis and therapy of M. pneumoniae infections.Palavras-chave
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Pneumonia por Mycoplasma
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Atenção Primária à Saúde
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Kit de Reagentes para Diagnóstico
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Atenção Terciária à Saúde
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RNA Ribossômico 23S
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Nasofaringe
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Macrolídeos
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Farmacorresistência Bacteriana
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Reação em Cadeia da Polimerase em Tempo Real
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Antibacterianos
Tipo de estudo:
Diagnostic_studies
Limite:
Child, preschool
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Female
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Humans
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Infant
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Male
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Newborn
Idioma:
En
Revista:
Annals of Laboratory Medicine
Ano de publicação:
2013
Tipo de documento:
Article