T Cell Immunoglobulin Mucin Domain (TIM)-3 Promoter Activity in a Human Mast Cell Line
Immune Network
; : 207-212, 2012.
Article
em En
| WPRIM
| ID: wpr-226024
Biblioteca responsável:
WPRO
ABSTRACT
T cell immunoglobulin mucin domain (TIM)-3 is an immunomodulatory molecule and upregulated in T cells by several cytokines. TIM-3 also influences mast cell function but its transcriptional regulation in mast cells has not been clarified. Therefore, we examined the transcript level and the promoter activity of TIM-3 in mast cells. The TIM-3 transcript level was assessed by real-time RT-PCR and promoter activity by luciferase reporter assay. TIM-3 mRNA levels were increased in HMC-1, a human mast cell line by TGF-beta1 stimulation but not by stimulation with interferon (IFN)-alpha, IFN-lambda, TNF-alpha, or IL-10. TIM-3 promoter -349~+144 bp region relative to the transcription start site was crucial for the basal and TGF-beta1-induced TIM-3 promoter activities in HMC-1 cells. TIM-3 promoter activity was increased by overexpression of Smad2 and Smad4, downstream molecules of TGF-beta1 signaling. Our results localize TIM-3 promoter activity to the region spanning -349 to +144 bp in resting and TGF-beta1 stimulated mast cells.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Imunoglobulinas
/
RNA Mensageiro
/
Linfócitos T
/
Citocinas
/
Interferons
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Fator de Necrose Tumoral alfa
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Interleucina-10
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Sítio de Iniciação de Transcrição
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Fator de Crescimento Transformador beta1
/
Luciferases
Limite:
Humans
Idioma:
En
Revista:
Immune Network
Ano de publicação:
2012
Tipo de documento:
Article