Changes in Caspase-3, B Cell Leukemia/Lymphoma-2, Interleukin-6, Tumor Necrosis Factor-alpha and Vascular Endothelial Growth Factor Gene Expression after Human Umbilical Cord Blood Derived Mesenchymal Stem Cells Transfusion in Pulmonary Hypertension Rat M
Korean Circulation Journal
; : 79-92, 2016.
Article
em En
| WPRIM
| ID: wpr-22787
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND AND OBJECTIVES:
Failure of vascular smooth muscle apoptosis and inflammatory response in pulmonary arterial hypertension (PAH) is a current research focus. The goals of this study were to determine changes in select gene expressions in monocrotaline (MCT)-induced PAH rat models after human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) transfusion. MATERIALS ANDMETHODS:
The rats were separated into 3 groups i.e., control group (C group), M group (MCT 60 mg/kg), and U group (hUCB-MSCs transfusion) a week after MCT injection.RESULTS:
TUNEL assay showed that the U group had significantly lowered positive apoptotic cells in the lung tissues, as compared with the M group. mRNA of caspase-3, B cell leukemia/lymphoma (Bcl)-2, interleukin (IL)-6, tumor necrosis factor (TNF)-alpha and vascular endothelial growth factor (VEGF) in the lung tissues were greatly reduced at week 4 in the U group. Immunohistochemical staining of the lung tissues also demonstrated a similar pattern, with the exception of IL-6. The protein expression of caspase-3, Bcl-2 VEGF, IL-6, TNF-alpha and brain natriuretic peptide in the heart tissues were significantly lower in the U group, as compared with the M group at week 2. Furthermore, the protein expression of VEGF, IL-6 and BNP in the heart tissues were significantly lower in the U group at week 4. Collagen content in the heart tissues was significantly lower in the U group, as compared with M group at weeks 2 and 4, respectively.CONCLUSION:
hUCB-MSCs could prevent inflammation, apoptosis and remodeling in MCT-induced PAH rat models.Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Células-Tronco
/
Cordão Umbilical
/
RNA Mensageiro
/
Expressão Gênica
/
Colágeno
/
Interleucinas
/
Interleucina-6
/
Fator de Necrose Tumoral alfa
/
Monocrotalina
/
Apoptose
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Korean Circulation Journal
Ano de publicação:
2016
Tipo de documento:
Article