Construction and identification of a vector inserted with gene of T7 RNA polymerase / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 146-148, 2011.
Article
em Zh
| WPRIM
| ID: wpr-231166
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a system to rescue virus by intracellular expression of T7 RNA Polymerase.</p><p><b>METHODS</b>The gene of T7 RNA Polymerase was amplified and cloned to VR1012 by molecular biological technology. The expression plasmid VR-1a was then identified. VR-1a and EV71 infectious plasmid were co-transfected in Vero cell. CPE was observed and viral gene viral antigen were detected.</p><p><b>RESULTS</b>The gene of T7 RNA Polymerase was successfully cloned into vector VR1012. Vero cell developed to CPE after being transfected VR-1a and EV71 infectious plasmid. EV71 gene was amplified by RT-PCR from the culture. EV71 antigen was also detected by ELISA.</p><p><b>CONCLUSION</b>The method can be used to rescue virus. It could apply to immunologic research of EV71 DNA vaccine.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fisiologia
/
Plasmídeos
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Proteínas Virais
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Replicação Viral
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Células Vero
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RNA Polimerases Dirigidas por DNA
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Células HeLa
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Transfecção
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Engenharia Genética
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Expressão Gênica
Limite:
Animals
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2011
Tipo de documento:
Article