RNA interference inhibits the expression of survivin and induces the apoptosis of PC-3 cells / 中华男科学杂志
National Journal of Andrology
; (12): 983-987, 2007.
Article
em Zh
| WPRIM
| ID: wpr-232027
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibitory effect of RNA interference (RNAi) on the expression of survivin mRNA and its inducibility of the apoptosis of PC-3 cells.</p><p><b>METHODS</b>siRNA expression vectors were designed and constructed to be directed at survivin and transfected into PC-3 cells by Lipofectamine in 4 groups plasmid A, plasmid B, negative sequence and control E. RT-PCR, Western blot and flow cytometry were used to detect the expression of survivin mRNA and the apoptosis of PC-3 cells.</p><p><b>RESULTS</b>The expression rates of survivin protein were 18.94% +/- 0.63%, 16.35 +/- 0.23%, 46.41% +/- 0.76% and 46.20 +/- 1.47%, those of survivin mRNA were 27.94% +/- 1.43%, 24.51% +/- 1.37%, 49.46% +/- 0.71% and 48.49% +/- 1.32%, and the apoptosis rates of PC-3 cells were 12.80% +/- 1.33%, 16.48% +/- 1.00%, 3.03% +/- 0.62% and 2.96% +/- 0.41% respectively in different groups.</p><p><b>CONCLUSION</b>RNAi can effectively inhibit the expression of survivin mRNA and induce the apoptosis of PC-3 cells.</p>
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Base de dados:
WPRIM
Assunto principal:
Patologia
/
Plasmídeos
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Neoplasias da Próstata
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RNA Mensageiro
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Transfecção
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Regulação Neoplásica da Expressão Gênica
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Química
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Apoptose
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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RNA Interferente Pequeno
Limite:
Humans
/
Male
Idioma:
Zh
Revista:
National Journal of Andrology
Ano de publicação:
2007
Tipo de documento:
Article