Differential gene expression profiling for identification of protective transcription factors in different subtypes of nasopharyngeal carcinoma / 南方医科大学学报
Journal of Southern Medical University
; (12): 1565-1570, 2013.
Article
em Zh
| WPRIM
| ID: wpr-232749
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the dysregulated genes among the differentially expressed genes in 41 nasopharyngeal biopsy samples and identify their protective transcriptional factors.</p><p><b>METHODS</b>The differentially expressed gene profiles were obtained by analyzing both types I and II nasopharyngeal carcinoma (NPC_I and NPC_II, respectively) using EXCEL and Bioinformatics tools. The transcriptional factors were further studied only when (1) the difference in the binding sites of the differentially expressed genes between NPC_I and NPC_II groups was statistically significant, (2) the expressions of the transcription factors were correlated with the gene expressions in the samples, and (3) the transcription factors affected at least 40% of the expression of the related genes.</p><p><b>RESULTS</b>In NPC_I samples, 80 transcription factors were found to be up-regulated, in which RUNX3, GATA3, NR3C1, NRF1, RXRA, SMAD7, TBP, and ZBTB6 were positive factors and HLF and MTF1 were negative factors, involved in the regulation of the genes in T cell receptor signaling pathway. No eligible transcription factors were found in association with down-regulated genes in NPC_I compared to NPC_II gene expression profiles.</p><p><b>CONCLUSIONS</b>The over-expressed genes in NPC_I are mainly related to immune responses, and we found 8 positive factors and 2 negative factors that regulate the genes in T cell receptor signaling pathway. The 10 transcription factors may serve as potential therapeutic targets for NPC_I. We failed to identify any transcription factors associated with down-regulated genes in NPC_I relative to NPC_II possibly as a result of multiple factors that affect the differential gene expressions in NPC_II including the transcription factors, DNA phosphorylation and modification, chromosome variation and environmental factors.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Patologia
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Fatores de Transcrição
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Carcinoma
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Receptores de Antígenos de Linfócitos T
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Transdução de Sinais
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Regulação Neoplásica da Expressão Gênica
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Neoplasias Nasofaríngeas
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Classificação
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Perfilação da Expressão Gênica
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Genética
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2013
Tipo de documento:
Article