Improved lipid productivity of Nannochloropsis by heavy-ion irradiation mutagenesis / 生物工程学报
Chinese Journal of Biotechnology
; (12): 119-122, 2013.
Article
em Zh
| WPRIM
| ID: wpr-233262
Biblioteca responsável:
WPRO
ABSTRACT
Nannochloropsis has been considered as a promising feedstock for biodiesel production in recent years. To improve its lipid productivity, heavy-ion irradiation mutagenesis, an effectively breeding method used in plants and microorganisms was applied in Nannochloropsis oceanica OZ-1. After large-scale screening using Imaging-PAM and microplate-reader, two mutants (HP-1 and HP-2) with higher growth rate were isolated from the wild type N. oceanica. Subsequently analysis showed that after 18 days of cultivation biomass accumulation of the HP-1 and HP-2 mutant was increased by 18% and 26% respectively compare to the wild type. Total lipid productivity of the HP-1 and HP-2 mutant was 295 mg/(L x d) and 275 mg/(L x d), respectively, whereas that of the wild type was 247 mg/(L x d). Both mutants showed significantly advantage over their wild type concerning biomass accumulation and lipid productivity.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Efeitos da Radiação
/
Mutagênese
/
Íons Pesados
/
Estramenópilas
/
Microalgas
/
Genética
/
Lipídeos
/
Metabolismo
/
Mutação
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2013
Tipo de documento:
Article