Studies on HPLC-fingerprint of Rhizoma Fagopyri Dibotoryis / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish an HPLC fingerprint of Rhizoma fagopyri dibotoryis.</p><p><b>METHOD</b>The HPLC-electrochemical detection assay was used to establish the fingerprint of Rhizoma fagopyri dibotoryis. The sample was performed on a column of Diamonsil C18 (4.6 mm x 250 mm, 5 microm) which was eluted with methanol- 0.1 mol x L(-1) phosphate buffer (pH 2.5), the flow rate was 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the work electrode was glassy carbon, the counter electrode was Pt platmun.</p><p><b>RESULT</b>The HPLC fingerprint profiles of 6 Rhizoma fagopyri dibotoryis contains 6 common chromatographic peaks, and gallic acid, protocatechuic acid, protocatechuic aldehyde and ( - ) -epicatechin were tested the samples. The contents of protocatechuic acid and protocatechuic aldehyde were from 0.004% to 0.05% and from 0.003% to 0.015%, respectively.</p><p><b>CONCLUSION</b>The method can be used to control the quality of Rhizoma fagopyri dibotoryis.</p>