Determination of hydromorphone in Beagle dogs plasma by liquid chromatography-tandem mass spectrometry / 药学学报
Acta Pharmaceutica Sinica
; (12): 929-932, 2004.
Article
em Zh
| WPRIM
| ID: wpr-241408
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To establish a LC/MS/MS method for determination of hydromorphone (HYD) in Beagle dog plasma.</p><p><b>METHODS</b>After incubation with beta-glucuronidase for 16 h, an aliquot of 0.1 mL plasma was treated by liquid-liquid extraction. The analytes of interest were separated on a Zorbax SB C8 column with the mobile phase consisting of methanol-water- formic acid (65: 35: 1). Atmospheric pressure chemical ionization source of MS was applied and operated in positive ion mode.</p><p><b>RESULTS</b>The linear calibration curve was obtained in the concentration range of 0.80 - 200.0 microg x L(-1). The lower limit of quantification was 0.80 microg x L(-1). The inter-day and intra-day precision (RSD) was below 6.0%, and the accuracy (RE) was within 1% calculated from QC samples. The method was used to determine the pharmacokinetic parameters of HYD after a single oral administration of 4 mg HYD sustained release tablets to Beagle dogs.</p><p><b>CONCLUSION</b>The method was proved to be specific, sensitive, and suitable for the pharmacokinetic study of HYD sustained release formulation.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Sangue
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Farmacocinética
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Reprodutibilidade dos Testes
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Cromatografia Líquida
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Sensibilidade e Especificidade
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Espectrometria de Massas por Ionização por Electrospray
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Preparações de Ação Retardada
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Hidromorfona
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Métodos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2004
Tipo de documento:
Article