Establishment of multiple regression model for virulence factors of Saccharomyces albicans by random amplified polymorphic DNA bands / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 643-645, 2011.
Article
em Zh
| WPRIM
| ID: wpr-241882
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To research the relationship between the virulence factors of Saccharomyces albicans (S. albicans) and the random amplified polymorphic DNA (RAPD) bands of them, and establish the regression model by multiple regression analysis.</p><p><b>METHODS</b>Extracellular phospholipase, secreted proteinase, ability to generate germ tubes and adhere to oral mucosal cells of 92 strains of S. albicans were measured in vitro; RAPD-polymerase chain reaction (RAPD-PCR) was used to get their bands. Multiple regression for virulence factors of S. albicans and RAPD-PCR bands was established.</p><p><b>RESULTS</b>The extracellular phospholipase activity was associated with 4 RAPD bands 350, 450, 650 and 1 300 bp (P < 0.05); secreted proteinase activity of S. albicans was associated with 2 bands 350 and 1 200 bp (P < 0.05); the ability of germ tube produce was associated with 2 bands 400 and 550 bp (P < 0.05).</p><p><b>CONCLUSION</b>Some RAPD bands will reflect the virulence factors of S. albicans indirectly. These bands would contain some important messages for regulation of S. albicans virulence factors.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Saccharomyces
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DNA
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Reação em Cadeia da Polimerase
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Técnica de Amplificação ao Acaso de DNA Polimórfico
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Fatores de Virulência
Tipo de estudo:
Clinical_trials
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Prognostic_studies
Idioma:
Zh
Revista:
West China Journal of Stomatology
Ano de publicação:
2011
Tipo de documento:
Article
País de publicação:
CHINA
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CN
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REPUBLIC OF CHINA