Effect of co-expression of nicotinic acid phosphoribosyl transferase and pyruvate carboxylase on succinic acid production in Escherichia coli BA002 / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1855-1859, 2013.
Article
em Zh
| WPRIM
| ID: wpr-242446
Biblioteca responsável:
WPRO
ABSTRACT
Escherichia coli BA002, in which the ldhA and pflB genes are deleted, cannot utilize glucose anaerobically due to the inability to regenerate NAD+. To restore glucose utilization, overexpression of nicotinic acid phosphoribosyltransferase (NAPRTase) encoded by the pncB gene, a rate-limiting enzyme of NAD(H) synthesis pathway, resulted in a significant increase in cell mass and succinate production under anaerobic conditions. However, a high concentration of pyruvate was accumulated. Thus, co-expression of NAPRTase and the heterologous pyruvate carboxylase (PYC) of Lactococcus lactis subsp. cremoris NZ9000 in recombinant E. coli BA016 was investigated. Results in 3 L fermentor showed that OD600 is 4.64 and BA016 consumed 35.00 g/L glucose and produced 25.09 g/L succinate after 112 h under anaerobic conditions. Overexpression of pncB and pyc in BA016, the accumulation of pyruvic acid was further decreased, and the formation of succinic acid was further increased.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Pentosiltransferases
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Piruvato Carboxilase
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Microbiologia Industrial
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Engenharia Genética
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Lactococcus lactis
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Ácido Succínico
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Escherichia coli
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Fermentação
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Genética
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Glucose
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2013
Tipo de documento:
Article