A preliminary genetic reassortment between Hantaan virus and Seoul virus strains / 中华流行病学杂志
Chinese Journal of Epidemiology
; (12): 46-49, 2002.
Article
em Zh
| WPRIM
| ID: wpr-244302
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the frequency and characteristics of reassortment among Hantaan and Seoul viruses causing hemorrhagic fever with renal syndrome (HFRS).</p><p><b>METHODS</b>Mixed infections were initiated in tissue culture, using Hantaan virus strain 76 - 118 and Seoul virus strain SR-11. Potential reassortant virus plaques were picked out by multiplex RT-PCR, using primers specific for individual genome segments (L, M, S) of each strain.</p><p><b>RESULTS</b>Most of the progeny virus plaques (68.19% of 44) had parental genotype of 76 - 118 strain or SR-11 strain while 2 of 44 plaques had mixed genotypes that yielded RT-PCR bands for the same segment of both parental strains. Reassortant viruses were detected in 68.19% of 44 progeny plaques tested, involving the M and S segments. In addition, approximately 4.55% of the progeny virus plaques appeared to contain S or M segments originating from both parental virus strains, showing that they were diploid.</p><p><b>CONCLUSION</b>Genetic reassortment can occur between Hantaan virus and Seoul virus strains.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Células Vero
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RNA Viral
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Chlorocebus aethiops
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Vírus Reordenados
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Genoma Viral
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Vírus Hantaan
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Vírus Seoul
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Genética
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Genótipo
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Epidemiology
Ano de publicação:
2002
Tipo de documento:
Article