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Determination of bufothionine in skin of Bufo bufo gargarizans and Huachansu injection / 中国中药杂志
Zhongguo Zhong Yao Za Zhi ; (24): 224-226, 2007.
Article em Zh | WPRIM | ID: wpr-246004
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a HPLC method for bufothionine in the skin of Bufo bufo gargarizans and Huachansu injection.</p><p><b>METHOD</b>The samples were separated using a Lichrosob C18 column with CH3CN-H2O (10:90) as mobile phase. Flow rate was at 1.0 mL x min (-1) and the detection wavelength was at 225 nm.</p><p><b>RESULT</b>The calibration curve of bufothionine was linear over the range of 0.0772-0.4632 microg and the average recovery was 99. 2%. The contents of bufothionine were fluctuated from 36.4-641.8 microg x g(-1) in the skin of Bufo bufo gargarizans and 22.47-33.16 microg x mL(-1) in Huachansu injection, respectively.</p><p><b>CONCLUSION</b>The contents of bufothionine were greatly different between cultured and wild species. The method was suitable for the quality control of the skin of Bufo bufo gargarizans and its preparation.</p>
Assuntos
Texto completo: 1 Base de dados: WPRIM Assunto principal: Fenotiazinas / Controle de Qualidade / Pele / Bufo bufo / Materia Medica / Química / China / Reprodutibilidade dos Testes / Cromatografia Líquida de Alta Pressão / Injeções Limite: Animals País/Região como assunto: Asia Idioma: Zh Revista: Zhongguo Zhong Yao Za Zhi Ano de publicação: 2007 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Assunto principal: Fenotiazinas / Controle de Qualidade / Pele / Bufo bufo / Materia Medica / Química / China / Reprodutibilidade dos Testes / Cromatografia Líquida de Alta Pressão / Injeções Limite: Animals País/Região como assunto: Asia Idioma: Zh Revista: Zhongguo Zhong Yao Za Zhi Ano de publicação: 2007 Tipo de documento: Article